Park Enoch Y, Sato Masayasu, Kojima Seiji
Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
Bioresour Technol. 2008 May;99(8):3130-5. doi: 10.1016/j.biortech.2007.05.059. Epub 2007 Jul 12.
The production of fatty acid methyl esters (FAMEs) from waste activated bleaching earth (ABE) discarded by the crude oil refining industry using lipase from Candida cylindracea was investigated in a 50-L pilot plant. Diesel oil or kerosene was used as an organic solvent for the transesterification of triglycerides embedded in the waste ABE. When 1% (w/w) lipase was added to waste ABE, the FAME content reached 97% (w/w) after reaction for 12 h at 25 degrees C with an agitation rate of 30 rpm. The FAME production rate was strongly dependent upon the amount of enzyme added. Mixtures of FAME and diesel oil at ratios of 45:55 (BDF-45) and 35:65 (BDF-35) were assessed and compared with the European specifications for biodiesel as automotive diesel fuel, as defined by pr EN 14214. The biodiesel quality of BDF-45 met the EN 14214 standard. BDF-45 was used as generator fuel, and the exhaust emissions were compared with those of diesel oil. The CO and SO2 contents were reduced, but nitrogen oxide emission increased by 10%. This is the first report of a pilot plant study of lipase-catalyzed FAME production using waste ABE as a raw material. This result demonstrates a promising reutilization method for the production of FAME from industrial waste resources containing vegetable oils for use as a biodiesel fuel.
在一个50升的中试装置中，研究了利用来自柱形假丝酵母的脂肪酶，将原油精炼行业丢弃的废活性白土（ABE）转化为脂肪酸甲酯（FAME）。柴油或煤油用作有机溶剂，用于废ABE中甘油三酯的酯交换反应。当向废ABE中添加1%（w/w）的脂肪酶时，在25℃、搅拌速率为30转/分钟的条件下反应12小时后，FAME含量达到97%（w/w）。FAME的产率强烈依赖于添加的酶量。评估了FAME与柴油比例为45:55（BDF - 45）和35:65（BDF - 35）的混合物，并与pr EN 14214定义的欧洲汽车柴油生物柴油规格进行了比较。BDF - 45的生物柴油质量符合EN 14214标准。BDF - 45用作发电机燃料，并将其废气排放与柴油的废气排放进行了比较。CO和SO₂含量降低，但氮氧化物排放增加了10%。这是首次关于以废ABE为原料，利用脂肪酶催化生产FAME的中试装置研究报告。该结果证明了一种从含有植物油的工业废料资源中生产FAME用作生物柴油燃料的有前景的再利用方法。
