Roeytenberg Annina, Cohen Tzeela, Freund Herbert R, Hanani Menachem
Department of Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
Nutrition. 2007 Sep;23(9):681-6. doi: 10.1016/j.nut.2007.06.004. Epub 2007 Jul 16.
Soybeans have been shown to have numerous health benefits, but the underlying mechanisms are poorly understood. The aim of this study was to characterize some pharmacologic properties of active substances in aqueous soy extract.
The pharmacologic actions of the extract were tested by measuring mechanical activity of isolated guinea-pig ileum in an organ bath.
The ileum contracted in response to soy extract in a concentration-dependent manner. This response was unaffected by the nerve blocker tetrodotoxin (10(-6) M) but was completely inhibited by atropine (10(-9) M), indicating an action via muscarinic receptors on the muscle. In the presence of the M(3) muscarinic antagonist 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide and to a lesser extent in the presence of the M(2) muscarinic antagonist 11-([2-[(diethylamino)methyl]-1-piperidinyl]acetyl)-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepine-6-one, the response was decreased. When acetylcholine (ACh) esterase inhibitors were added to the medium before the addition of soy extract, the response to the extract was potentiated. Preincubation of the extract with exogenous ACh esterase reduced its activity. The response to choline, ACh, and phosphorylcholine was also tested, and none of these substances accurately replicated the response to soy extract. However, some qualitative similarities were observed between the effect of choline and ACh to that of the extract.
These results indicate the presence of an ACh-like substance in soy. Due to the abundance and importance of muscarinic receptors, the presence of a cholinergic substance in soy could have numerous implications. The role of this substance in the beneficial effect of soy on various body systems merits further investigation.
大豆已被证明具有众多健康益处，但其潜在机制尚不清楚。本研究的目的是表征大豆水提取物中活性物质的一些药理特性。
通过在器官浴中测量分离的豚鼠回肠的机械活性来测试提取物的药理作用。
回肠对大豆提取物的收缩反应呈浓度依赖性。这种反应不受神经阻滞剂河豚毒素（10^(-6) M）的影响，但被阿托品（10^(-9) M）完全抑制，表明其作用是通过作用于肌肉上的毒蕈碱受体。在存在M(3)毒蕈碱拮抗剂1,1-二甲基-4-二苯基乙酰氧基碘化哌啶的情况下，以及在较小程度上在存在M(2)毒蕈碱拮抗剂11-([2-[(二乙氨基)甲基]-1-哌啶基]乙酰基)-5,11-二氢-6H-吡啶并[2,3-b][1,4]苯并二氮杂卓-6-酮的情况下，反应减弱。当在添加大豆提取物之前向培养基中添加乙酰胆碱（ACh）酯酶抑制剂时，对提取物的反应增强。提取物与外源性ACh酯酶预孵育会降低其活性。还测试了对胆碱、ACh和磷酰胆碱的反应，这些物质均未准确复制对大豆提取物的反应。然而，观察到胆碱和ACh的作用与提取物的作用之间存在一些定性相似性。
这些结果表明大豆中存在一种类似ACh的物质。由于毒蕈碱受体的丰富性和重要性，大豆中胆碱能物质的存在可能具有众多影响。该物质在大豆对各种身体系统的有益作用中的作用值得进一步研究。
