Sun Hongxiang, Pan Hangjun, Yang Zhigang, Shi Minghua
College of Animal Sciences, Zhejiang University, Kaixuan Road 268, Hangzhou, Zhejiang, 310029, People's Republic of China.
Int Immunopharmacol. 2007 Sep;7(9):1259-64. doi: 10.1016/j.intimp.2007.05.003. Epub 2007 May 25.
The effect of an oral alginate microparticle Aeromonas sobria vaccine on immune response and protection of mice was investigated. The formalin-killed cultures (FKC) of A. sobria strain Z-1 treated with three methods were incorporated into alginate microparticles, and the encapsulated vaccines were orally used for immunizing mice. Four weeks after immunization, the serum agglutinating antibody titers, bactericidal activity of leucocytes and relative percent survival (RPS) of mice immunized orally with three microparticle vaccines were significantly higher than those of control mice. Among the immune efficacy of 3 microparticle vaccines, vaccine prepared directly from FKC (MVC) was the most, vaccine from FKC treated with freeze-melt method (MVCF) secondly, and vaccine from FKC treated with ultrasonic wave (MVCU) the worst. The serum agglutinating antibody titers and phagocytic activity of the blood monocytes in mice immunized orally with MVC reached a level equivalent to those of injection with FKC, and were significantly higher than those of control group. Moreover, from the 7th to 12th weeks, the serum agglutinating antibody titers in the MVC-immunized mice was significantly higher than those of injection with FKC. The RPS of mice immunized orally with MVC was 87.5%, while 100% of mice immunized orally with FKC and also the control mice died. These results demonstrated that oral alginate microparticle of A. sobria strain Z-1 was immunogenic and conferred protective immunity in mice, and could be used as a candidate vector system of oral vaccine of Aeromonas.
研究了口服海藻酸盐微粒嗜水气单胞菌疫苗对小鼠免疫反应和保护作用的影响。将用三种方法处理的嗜水气单胞菌Z-1株福尔马林灭活培养物（FKC）掺入海藻酸盐微粒中，将包封的疫苗口服用于免疫小鼠。免疫四周后，用三种微粒疫苗口服免疫的小鼠血清凝集抗体效价、白细胞杀菌活性和相对存活率（RPS）均显著高于对照小鼠。在三种微粒疫苗的免疫效果中，直接由FKC制备的疫苗（MVC）效果最佳，冻融法处理的FKC制备的疫苗（MVCF）次之，超声波处理的FKC制备的疫苗（MVCU）最差。口服MVC免疫的小鼠血清凝集抗体效价和血液单核细胞吞噬活性达到与注射FKC相当的水平，且显著高于对照组。此外，在第7至12周，口服MVC免疫的小鼠血清凝集抗体效价显著高于注射FKC的小鼠。口服MVC免疫的小鼠RPS为87.5%，而口服FKC免疫的小鼠和对照小鼠全部死亡。这些结果表明，嗜水气单胞菌Z-1株口服海藻酸盐微粒具有免疫原性，并能在小鼠中提供保护性免疫，可作为嗜水气单胞菌口服疫苗的候选载体系统。
