James Morré D, Mollenhauer Hilton H
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
Int Rev Cytol. 2007;262:191-218. doi: 10.1016/S0074-7696(07)62004-X.
The membrane maturation (flow differentiation) model of Golgi apparatus function embodies concepts of saccule formation at one face of the Golgi apparatus from membranes derived from endoplasmic reticulum and utilization of saccules in vesicle formation at the opposite face for delivery to the plasma membrane as existing saccules are displaced from one position within the stack to another. Derivation of the model came almost entirely from light and electron microscopy. Especially important were observations that passage through the Golgi apparatus was accompanied by differentiation of membranes from endoplasmic reticulum-like to plasma membrane-like across the polarity axis of the stacked saccules. The concept of coparticipation of endoplasmic reticulum and/or nuclear envelope, transition, and secretory vesicles and other pre- and post-Golgi apparatus structures through the operation of an integrated endomembrane system was essential to the model. Dynamic aspects confirmed initially by autoradiographing and cell fractionation studies have been corroborated in newer approaches of fluorescent labeling and with living cells.
高尔基体功能的膜成熟（流动分化）模型体现了这样的概念：高尔基体一侧的扁平囊泡由内质网衍生的膜形成，而在另一侧，当现有扁平囊泡在堆叠中从一个位置移位到另一个位置时，扁平囊泡用于形成囊泡并输送到质膜。该模型几乎完全源自光学显微镜和电子显微镜观察。特别重要的是观察到，穿过高尔基体时，沿着堆叠扁平囊泡的极性轴，膜会从内质网样分化为质膜样。内质网和/或核膜、过渡囊泡和分泌囊泡以及高尔基体前后的其他结构通过整合内膜系统的运作而共同参与，这一概念对该模型至关重要。最初通过放射自显影和细胞分级分离研究证实的动态方面，在荧光标记和活细胞的新方法中得到了证实。
