Xu J L, Khor K A, Lu Y W, Chen W N, Kumar R
School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
J Biomed Mater Res B Appl Biomater. 2008 Jan;84(1):224-30. doi: 10.1002/jbm.b.30864.
This study investigated the osteoblast behaviors on various hydroxyapatite based biomaterials that were consolidated at 1100 degrees C for 3 min by a spark plasma sintering technique. The osteoblasts from human fetal osteoblast cell line were cultured in the medium on the various biomaterials surfaces (HA, RF21, 1SiHA, and 5SiHA) to assess the cell morphology and proliferation as well as cell differentiation (alkaline phosphatase activity). Moreover, the bone gamma-carboxyglutamic protein or osteocalcin in the medium were determined at different periods of culture. The present results indicated that the amount of osteocalcin in the medium decreased during the periods of culture. The highest osteocalcin production obtained from the biomaterial 5SiHA after cell culture for 2 days demonstrated that the presence of silica in the biomaterials enhanced the cell differentiation by the rapid release of silicate and calcium ions.
本研究调查了通过火花等离子体烧结技术在1100摄氏度下固结3分钟的各种羟基磷灰石基生物材料上的成骨细胞行为。将来自人胎儿成骨细胞系的成骨细胞在各种生物材料表面（HA、RF21、1SiHA和5SiHA）的培养基中培养，以评估细胞形态、增殖以及细胞分化（碱性磷酸酶活性）。此外，在培养的不同时期测定培养基中的骨γ-羧基谷氨酸蛋白或骨钙素。目前的结果表明，培养基中骨钙素的量在培养期间减少。细胞培养2天后，从生物材料5SiHA获得的骨钙素产量最高，这表明生物材料中二氧化硅的存在通过硅酸盐和钙离子的快速释放增强了细胞分化。
