金黄色葡萄球菌肠毒素B对人鼻上皮细胞的促炎作用

[Proinflammatory effects of Staphylococcus aureus enterotoxin B on human nasal epithelial cells].

作者信息

Yu Rui-Li, Zhu Dong-Dong, Dong Zhen

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Mar;42(3):202-6.

PMID:17633280

Abstract

OBJECTIVE

To evaluate the effects of Staphylococcus aureus enterotoxin B (SEB) on proinflammatory cytokine/chemokine releases in primary human nasal epithelial cell (HNEC).

METHODS

Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of SEB 1, 10, 100 ng/ml, IL-1beta 20 ng/ml and SEB 10 ng/ml + dexamethasone 13 ng/ml for 12,24 and 48 h, respectively. The expression of IL-5 and Granulocyte-macrophage colony stimulating factor (GM-CSF) mRNA derived from epithelial cells was detected by in situ hybridization.

RESULTS

(1) The expression of IL-5 and GM-CSF mRNA was time and dose-dependent, and reached to a peak under SEB 10 ng/ml for 24 h (P < 0.05). The mRNA expressed more intensively in epithelial cells from NP than IT (P < 0.05). (2) The expression of IL-5 and GM-CSF mRNA increased less under the stimulus of IL-1beta than SEB 10 ng/ml (P < 0.05). (3) The mRNA level of IL-5 and GM-CSF decreased under the stimulus of SEB + dexamethasone 13 ng/m when compared with the stimulus of SEB 10 ng/ml (P < 0.05).

CONCLUSION

SEB showed proinflammatory effects on HNEC.

摘要

目的

评估金黄色葡萄球菌肠毒素B（SEB）对原代人鼻上皮细胞（HNEC）促炎细胞因子/趋化因子释放的影响。

方法

分别在无血清条件下，用1、10、100 ng/ml的SEB、20 ng/ml的IL-1β以及10 ng/ml的SEB + 13 ng/ml地塞米松刺激鼻息肉（NP）和下鼻甲（IT）的上皮细胞12、24和48小时。通过原位杂交检测上皮细胞来源的IL-5和粒细胞-巨噬细胞集落刺激因子（GM-CSF）mRNA的表达。

结果

（1）IL-5和GM-CSF mRNA的表达呈时间和剂量依赖性，在10 ng/ml的SEB刺激24小时时达到峰值（P < 0.05）。NP上皮细胞中mRNA的表达比IT上皮细胞更强烈（P < 0.05）。（2）IL-1β刺激下IL-5和GM-CSF mRNA表达的增加幅度小于10 ng/ml的SEB刺激（P < 0.05）。（3）与10 ng/ml的SEB刺激相比，SEB + 13 ng/ml地塞米松刺激下IL-5和GM-CSF的mRNA水平降低（P < 0.05）。