[人鼻上皮细胞中白细胞介素12、细胞间黏附分子1及单核细胞趋化蛋白3的mRNA水平特征]
[Characteristics of mRNA level of interleukin 12 and intercellular adhesion molecule 1 and monocyte chemotactic protein 3 in human nasal epithelial cells].
作者信息
Zhu Xuewei, Zhu Dongdong, Sun Kewei, Dong Zhen
机构信息
Department of Otolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Dec;22(23):1068-70.
OBJECTIVE
To investigate the mRNA level of IL-12, ICAM-1 and MCP-3 in human nasal epithelial cells.
METHOD
Firstly, human primary nasal epithelial cells were cultured, and then 4 pairs of primers were designed for detecting mRNA level of IL-12, ICAM-1 and MCP-3. The 938 bp PCR products of GAPDH were used as internal standards. The mRNA expression levels of IL-12, ICAM-1 and MCP-3 in primary nasal epithelial cells was measured with semi-quantitative reverse transcription-polymerase chain reaction.
RESULT
The round or irregular primary nasal epithelial cells were observed sticking to the bottom of cell culture plates under 400 times optical microscope. The expressions of IL-12 p35, ICAM-1 and MCP-3 mRNA were found in primary nasal epithelial cells while IL-12 p40 subunit was not detected.
CONCLUSION
IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells, whereas effective IL-12 with integrity is not present in nasal epithelial cells.
目的
研究人鼻上皮细胞中白细胞介素12（IL-12）、细胞间黏附分子1（ICAM-1）和单核细胞趋化蛋白3（MCP-3）的信使核糖核酸（mRNA）水平。
方法
首先培养人原代鼻上皮细胞，然后设计4对引物用于检测IL-12、ICAM-1和MCP-3的mRNA水平。甘油醛-3-磷酸脱氢酶（GAPDH）的938碱基对聚合酶链反应（PCR）产物用作内参。采用半定量逆转录-聚合酶链反应检测原代鼻上皮细胞中IL-12、ICAM-1和MCP-3的mRNA表达水平。
结果
在400倍光学显微镜下观察到圆形或不规则形的原代鼻上皮细胞贴附于细胞培养板底部。在原代鼻上皮细胞中发现了IL-12 p35、ICAM-1和MCP-3 mRNA的表达，而未检测到IL-12 p40亚基。
结论
IL-12 p35、ICAM-1和MCP-3的mRNA在原代鼻上皮细胞中表达，而鼻上皮细胞中不存在完整有效的IL-12。
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