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[从天然土壤中分离苯酚降解菌及其系统发育分析]

[Isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis].

作者信息

Wang Yun-Duan, Dong Xiao-Jun, Wang Xi, Hong Qing, Jiang Xin, Li Shun-Peng

机构信息

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

出版信息

Huan Jing Ke Xue. 2007 Mar;28(3):623-6.

Abstract

Five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. They were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. Strain PHD-2, PHD-4 and PHD-5 belonged to the genera of Ralstonia, Acinetobacter and Microbacterium respectively; strain PHD-1 and PHD-3 were from the genus of Pseudomonas. Homology comparing of their 16S rRNA gene sequences and phylogenetic analysis displayed the high biodiversity of phenol-degrading microorganisms in the natural soil.

摘要

从在苯酚存在下富集培养的未受污染天然土壤样品中分离出五株能够利用苯酚作为唯一碳源生长的细菌菌株。根据它们的系统发育分析以及生理生化特性对其进行了初步鉴定。菌株PHD - 2、PHD - 4和PHD - 5分别属于罗尔斯通氏菌属、不动杆菌属和微杆菌属;菌株PHD - 1和PHD - 3属于假单胞菌属。对它们的16S rRNA基因序列进行同源性比较和系统发育分析,显示出天然土壤中苯酚降解微生物具有高度的生物多样性。

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