Ndione R D, Sèye F, Ndiaye M, Sow A I
Université Cheikh Anta Diop de Dakar, Département de Biologie Animale, Laboratoire de Biologie de la Reproduction, Unité d'Entomologie, de Rickettsiologie, de Bactériologie et de Virologie, BP 5005 Dakar, Sénégal.
Dakar Med. 2006;51(2):107-12.
Histopathological studies relating to last instars larvae of mosquitoes treated with neem products are very few. The objective were to study the action of Neem products on larvae mosquitoes of Culex quinquefasciatus.
The neem products (Neemix, Formulated neem oil, Pure neem oil and Neem powder) are tested on 4 instars larvae of Culex quinquefasciatus. Moribund and witness larvae were fixed, for histopathologic study.
The results showed the following facts: an intestinal epithelium, a bross border, a fat body and others tissues are normals at the level of the untreated larva; Fat body and muscle are not touched during the treatment of the larva with Pure neem oil; Intestinal epithelial cells slightly hypertrophied with beaches of lysis on the level of the larva treated with Neem powder; a lysis of the microvilli of brush border on the level of the witness-solvent; a destruction of the epithelial cells recorded in larva treated with a Neemix; a disappearance of the food column and a destruction of the epithelial cells remarked on the level of the larva treated with Formulated neem oil.
The neem products used in this study have a Larvaecidal action on Culex quinquefasciatus larvae.
关于用印楝产品处理过的蚊子末龄幼虫的组织病理学研究非常少。目的是研究印楝产品对致倦库蚊幼虫的作用。
将印楝产品(印楝混合剂、配方印楝油、纯印楝油和印楝粉)用于致倦库蚊的4龄幼虫。濒死和对照幼虫被固定,用于组织病理学研究。
结果显示了以下事实:在未处理幼虫水平,肠上皮、刷状缘、脂肪体和其他组织正常;在用纯印楝油处理幼虫期间,脂肪体和肌肉未受影响;在用印楝粉处理的幼虫水平,肠上皮细胞轻度肥大且有溶解区;在对照溶剂处理水平,刷状缘微绒毛溶解;在用印楝混合剂处理的幼虫中记录到上皮细胞破坏;在用配方印楝油处理的幼虫水平,食物柱消失且上皮细胞破坏。
本研究中使用的印楝产品对致倦库蚊幼虫有杀幼虫作用。