Wright P F, Thomas W D, Stacey N H
National Institute of Occupational Health and Safety, University of Sydney, N.S.W., Australia.
Toxicology. 1991;70(2):231-42. doi: 10.1016/0300-483x(91)90049-7.
The effects of trichloroethylene (TRI), a widely used industrial solvent, on various immunological and toxicological parameters have been examined in Sprague-Dawley rats and B6C3F1 mice. Rats were administered TRI in vivo at 0.05, 0.5 and 5.0 mmol/kg per day intraperitoneally (i.p.) for 3 days. Mice were similarly treated with TRI at 10.0 mmol/kg. The highest TRI dose resulted in decreased splenocyte count and relative spleen weights, in rats and mice respectively and inhibition of hepatic natural killer cell (NK), natural cytotoxic cell (NC) and NPK cell (a newly described immune cell killing) activities in both rats and mice. High dose TRI in vitro resulted in minor decreases (less than 10%) in splenocyte viability, inhibition of LPS-stimulated mitogenesis in rat cells and marked inhibitions of NK and NC activities in all groups of effector cells. At the lowest in vitro dose mouse hepatic NK activity was still inhibited. Overall the data show that TRI is able to inhibit the activity of lymphocytotoxic cells which are involved in the immune surveillance of cancerous cells. This inhibition is particularly evident in the liver after in vivo administration and both liver and spleen cells after in vitro exposure. This suggests the possibility that compromised immune function may play a role in the carcinogenic responses in experimental animals on exposure to TRI.
三氯乙烯（TRI）是一种广泛使用的工业溶剂，其对各种免疫和毒理学参数的影响已在斯普拉格-道利大鼠和B6C3F1小鼠中进行了研究。大鼠腹腔内（i.p.）每天以0.05、0.5和5.0 mmol/kg的剂量给予TRI，持续3天。小鼠以10.0 mmol/kg的剂量给予类似处理。最高剂量的TRI分别导致大鼠和小鼠的脾细胞计数和相对脾脏重量下降，并抑制大鼠和小鼠肝脏自然杀伤细胞（NK）、自然细胞毒性细胞（NC）和NPK细胞（一种新描述的免疫细胞杀伤）的活性。高剂量的TRI在体外导致脾细胞活力略有下降（小于10%），抑制大鼠细胞中脂多糖刺激的有丝分裂，并显著抑制所有效应细胞组中的NK和NC活性。在最低的体外剂量下，小鼠肝脏NK活性仍受到抑制。总体而言，数据表明TRI能够抑制参与癌细胞免疫监视的淋巴细胞毒性细胞的活性。这种抑制在体内给药后在肝脏中尤为明显，在体外暴露后在肝脏和脾脏细胞中均有体现。这表明免疫功能受损可能在实验动物接触TRI后的致癌反应中起作用。
