Letellier C, Rameliarison L, Fizet D, Ferrer A M, Vezon G
Service d'immunologie cellulaire, CRTS de Bordeaux, France.
Vox Sang. 1991;61(2):90-5. doi: 10.1111/j.1423-0410.1991.tb00252.x.
Ficoll-separated and monocyte-depleted mononuclear cells isolated from normal leukapheresis products were cryopreserved. These cells were incubated with or without 1,000 U/ml of recombinant interleukin-2 (rIL-2) for 4 days, and their lymphokine-activated killer (LAK) and natural killer (NK) activities were measured. IL-2 activation induced a significant increase in the expression of the CD25 antigen. There was no change in CD2, CD3, CD4, CD8, CD16, CD56 and CD57 cell marker expression. Cryopreservation did not induce any change in the membrane antigen expression and in the lymphocyte subsets. The NK activity was well preserved and the decrease of LAK activity of IL-2-activated cells after cryopreservation was not significant. In contrast, cells activated before cryopreservation had a significantly lower cytotoxic activity and the number of cells expressing the IL-2 receptor was also significantly reduced. However, the decrease of CD56 expression was not significant. CD25 expression seemed to be proportional to the LAK activity of the cells. This study demonstrated that cryopreserved lymphocytes, after 4 days of culture with rIL-2, could be as active and could express the CD25 and CD56 cell surface markers in the same manner as fresh LAK cells.
从正常白细胞单采产品中分离出的经Ficoll分离和去除单核细胞的单个核细胞被冻存。将这些细胞在有或无1000 U/ml重组白细胞介素-2（rIL-2）的条件下培养4天，并检测其淋巴因子激活的杀伤细胞（LAK）和自然杀伤细胞（NK）活性。IL-2激活导致CD25抗原表达显著增加。CD2、CD3、CD4、CD8、CD16、CD56和CD57细胞标志物表达没有变化。冻存未诱导膜抗原表达和淋巴细胞亚群发生任何改变。NK活性得到良好保留，冻存后IL-2激活细胞的LAK活性下降不显著。相比之下，冻存前激活的细胞细胞毒性活性显著降低，表达IL-2受体的细胞数量也显著减少。然而，CD56表达的下降不显著。CD25表达似乎与细胞的LAK活性成正比。本研究表明，经rIL-2培养4天后的冻存淋巴细胞与新鲜LAK细胞一样活跃，并且能够以相同方式表达CD25和CD56细胞表面标志物。
