Munsi M N, Bhuiyan M M U, Majumder S, Alam M G S
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Reprod Domest Anim. 2007 Aug;42(4):358-62. doi: 10.1111/j.1439-0531.2006.00789.x.
Glutathione (GSH) at concentrations of 0.0 (control), 0.5, 1.0, 2.0 and 3.0 mM was added to chilled bull semen to determine its effects on the keeping quality of semen used for artificial insemination (AI). The semen was preserved with egg yolk citrate extender. All samples were stored at 4-8 degrees C for 5 days. Sperm motility and proportion of abnormal acrosome were assessed daily. Sperm motility was significantly (p < 0.01) higher in the semen treated with 0.5 mM glutathione than in untreated semen on each day. The optimum sperm motility (>or=50%) for AI was retained significantly (p < 0.01) for 3 days in 0.0, 0.5, 1.0 and 2.0 mM glutathione treated semen, whereas in 0.3 mM glutathione-treated semen, sperm motility was 46.8% for 3 days. Acrosomal damage was significantly (p < 0.01) reduced after addition of 0.5 mM GSH in the preserved semen. Bull semen can be preserved in chilled condition for 5 days with 0.5 mM GSH with sperm motility above 40% and 12% acrosome abnormality.
将浓度为0.0（对照）、0.5、1.0、2.0和3.0 mM的谷胱甘肽（GSH）添加到冷冻的公牛精液中，以确定其对用于人工授精（AI）的精液保存质量的影响。精液用蛋黄柠檬酸盐稀释液保存。所有样品在4-8摄氏度下储存5天。每天评估精子活力和顶体异常比例。在处理后的每一天，用0.5 mM谷胱甘肽处理的精液中的精子活力均显著高于未处理的精液（p<0.01）。在0.0、0.5、1.0和2.0 mM谷胱甘肽处理的精液中，AI的最佳精子活力（≥50%）显著保持3天（p<0.01），而在0.3 mM谷胱甘肽处理的精液中，精子活力在3天内为46.8%。在保存的精液中添加0.5 mM GSH后，顶体损伤显著降低（p<0.01）。公牛精液在添加0.5 mM GSH的冷藏条件下可保存5天，精子活力高于40%，顶体异常率为12%。
Zotero 插件