College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
Cryobiology. 2011 Feb;62(1):83-7. doi: 10.1016/j.cryobiol.2010.12.007. Epub 2011 Jan 1.
Egg low-density lipoprotein (LDL) was added at concentrations (w/v) of 7%, 8% or 9% to the extenders used to freeze bull semen and its effects on seminal parameters and anti-oxidant activities of frozen-thawed sperm were assessed. Analysis of data showed that sperm exposed to 8% LDL exhibited the greatest percentages of sperm motility, acrosome integrity and membrane integrity, compared to the control which differed from the treatment groups by replacing LDL with 20% egg yolk (P<0.05). No difference was observed for membrane integrity between 8% and 9% LDL groups (P>0.05). The extender supplemented with LDL did not exhibit improvement in SOD levels. However, 8% LDL group favored the highest anti-oxidant activities of CAT, GSH-Px and GSH in comparison to other groups (7%, 9% LDL and the control) (P<0.05). No difference was observed for CAT activity between 9% LDL and the control group. In conclusion, sperm cryopreserved in the extender containing 8% LDL in place of egg yolk exhibited the greatest percentages of post-thaw sperm motility, acrosome integrity and membrane integrity, in comparison with the control, and favored the highest anti-oxidant activities of CAT, GSH-Px and GSH in comparison with other groups. The replacement of egg yolk by LDL in the composition of extenders was beneficial for bull sperm cryopreservation.
将卵低密度脂蛋白 (LDL) 以 7%、8% 或 9%（w/v）的浓度添加到用于冷冻公牛精液的稀释液中，评估其对精液参数和冷冻解冻精子抗氧化活性的影响。数据分析表明，与对照组（用 20%卵黄代替 LDL）相比，暴露于 8% LDL 的精子表现出最大的精子运动能力、顶体完整性和膜完整性百分比，差异具有统计学意义（P<0.05）。8% 和 9% LDL 组之间的膜完整性没有差异（P>0.05）。添加 LDL 的稀释液并没有提高 SOD 水平。然而，与其他组（7%、9% LDL 和对照组）相比，8% LDL 组的 CAT、GSH-Px 和 GSH 的抗氧化活性最高（P<0.05）。9% LDL 组和对照组之间的 CAT 活性没有差异。总之，与对照组相比，用替代卵黄的含有 8% LDL 的稀释液冷冻的精子解冻后具有最高的精子运动能力、顶体完整性和膜完整性百分比，与其他组相比，CAT、GSH-Px 和 GSH 的抗氧化活性最高。在稀释液的组成中用 LDL 替代卵黄有利于公牛精子的冷冻保存。
