Mehmood A, Anwar M, Saqlan Naqvi S M
Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan.
Reprod Domest Anim. 2007 Aug;42(4):376-9. doi: 10.1111/j.1439-0531.2006.00794.x.
The present study was conducted to examine the effect of high heparin concentration on capacitation of buffalo spermatozoa with a short incubation time. Frozen thawed spermatozoa from three buffalo bulls were pooled and treated with either 50, 100 or 200 microg/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and in vitro fertilization rate (per cent cleavage rate, per cent cleavage index). Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with Coomassie G-250 to evaluate the response as compared with control (0 heparin + calcium ionophore). Significantly higher percentage of acrosome reaction (AR) spermatozoa was noted after heparin treatment (36.8-48.2%) as compared with control (8.1% ; p < 0.05) but differences among the three heparin concentrations were non-significant. However, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 microg/ml heparin (80.4 and 75.9% cleavage rate, respectively) as compared with 200 microg/ml heparin (47.2% cleavage rate; p < 0.001). It is concluded that buffalo spermatozoa capacitated with 50-100 microg/ml heparin had significantly higher ability to improve in vitro fertilization rate in buffalo.
本研究旨在考察高浓度肝素在短孵育时间下对水牛精子获能的影响。将来自三头水牛公牛的冻融精子混合，分别用50、100或200微克/毫升的肝素处理30分钟。通过精子顶体反应和体外受精率（卵裂率百分比、卵裂指数百分比）评估获能情况。用钙离子载体A23187诱导肝素处理的精子发生顶体反应，并用考马斯亮蓝G - 250染色，以评估与对照（0肝素 + 钙离子载体）相比的反应情况。与对照（8.1%；p < 0.05）相比，肝素处理后顶体反应（AR）精子的百分比显著更高（36.8 - 48.2%），但三种肝素浓度之间的差异不显著。然而，与200微克/毫升肝素（卵裂率47.2%；p < 0.001）相比，用50和100微克/毫升肝素获能的精子体外受精率显著更高（分别为80.4%和75.9%的卵裂率）。得出的结论是，用50至100微克/毫升肝素获能的水牛精子在提高水牛体外受精率方面具有显著更高的能力。
