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马周期性黄体中的孕酮与半胱天冬酶-3激活

Progesterone and caspase-3 activation in equine cyclic corpora lutea.

作者信息

Ferreira-Dias G, Mateus L, Costa A S, Solá S, Ramalho R M, Castro R E, Rodrigues C M P

机构信息

Faculdade de Medicina Veterinária, CIISA, Lisboa, Portugal.

出版信息

Reprod Domest Anim. 2007 Aug;42(4):380-6. doi: 10.1111/j.1439-0531.2006.00795.x.

Abstract

Soon after ovulation, the newly formed corpus luteum (CL) starts secreting progesterone (P(4)), necessary for implantation. The CL, an ovarian transient endocrine organ, undergoes growth and regression throughout its life span. The objective of this study was to evaluate if caspase-3 mediates cell death in the equine cyclic luteal structures and relate it to luteal endocrine function. Blood and luteal tissue were collected during the breeding season after slaughter from 38 randomly assigned cycling mares. Luteal tissues were classified as corpora haemorrhagica (CH; n = 7); mid luteal phase corpora lutea (Mid-CL; n = 17); late or regressing corpora lutea (Late-CL; n = 9) and corpora albicans (CA; n = 5). Plasma P(4) concentration, determined by radioimmunoassay, showed a significant increase from CH to Mid-CL (p < 0.001), followed by a decrease to Late-CL (p < 0.001) and CA (p < 0.001). Caspase-3 processing and poly (ADP) ribose polymerase (PARP) degradation were assessed by western blotting. Active caspase-3 was twofold increased in Mid-CL, Late-CL and CA as compared with CH (p < 0.05). Immunocytochemistry also showed a significant increase in caspase-3 expression in large luteal cells in all structures when compared with CH (p < 0.05). Consistently, the endogenous caspase-3 substrate, PARP, was markedly degraded from CH to CA (p < 0.05). In fact, the ratio of full-length to degraded PARP showed a significant decrease from CH to Mid-CL, Late-CL and CA (p < 0.05). Finally, the decrease in P(4) from Mid- to Late-CL coincided with no further increases in apoptosis. In conclusion, these results suggest that the effector caspase-3 of apoptosis, might play an important role during luteal tissue involution in the mare, even though its relationship with P(4) remains to be elucidated.

摘要

排卵后不久,新形成的黄体(CL)开始分泌植入所必需的孕酮(P(4))。黄体是卵巢的一个短暂内分泌器官,在其整个生命周期中经历生长和退化。本研究的目的是评估半胱天冬酶-3是否介导马周期性黄体结构中的细胞死亡,并将其与黄体内分泌功能相关联。在繁殖季节屠宰后,从38匹随机分配的处于发情周期的母马中采集血液和黄体组织。黄体组织分为出血性黄体(CH;n = 7);黄体中期黄体(Mid-CL;n = 17);晚期或退化黄体(Late-CL;n = 9)和白体(CA;n = 5)。通过放射免疫测定法测定的血浆P(4)浓度显示,从CH到Mid-CL显著升高(p < 0.001),随后降至Late-CL(p < 0.001)和CA(p < 0.001)。通过蛋白质印迹法评估半胱天冬酶-3的加工和聚(ADP)核糖聚合酶(PARP)的降解。与CH相比,Mid-CL、Late-CL和CA中的活性半胱天冬酶-3增加了两倍(p < 0.05)。免疫细胞化学也显示,与CH相比,所有结构中的大黄体细胞中半胱天冬酶-3的表达显著增加(p < 0.05)。一致地,内源性半胱天冬酶-3底物PARP从CH到CA明显降解(p < 0.05)。事实上,全长PARP与降解PARP的比率从CH到Mid-CL、Late-CL和CA显著降低(p < 0.05)。最后,从黄体中期到晚期P(4)的下降与细胞凋亡没有进一步增加同时发生。总之,这些结果表明,凋亡的效应半胱天冬酶-3可能在母马黄体组织退化过程中起重要作用,尽管其与P(4)的关系仍有待阐明

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