Gibbons A, Pereyra Bonnet F, Cueto M I, Catala M, Salamone D F, Gonzalez-Bulnes A
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Bariloche, Bariloche, Argentina.
Reprod Domest Anim. 2007 Aug;42(4):423-6. doi: 10.1111/j.1439-0531.2006.00802.x.
Possible effects of repeated hormonal treatments and laparoscopic ovum pick-up (LOPU) on the efficiency of oocyte recovery rate and quality were determined in sheep and goats. In six adult Merino sheep and five Criolla goats, ovarian status was synchronized by a prostaglandin F(2 alpha) analogue and the insertion of an intravaginal sponge 48 h later. Follicle development was stimulated by a single dose of FSH (60 mg NIH-FSH-P1) plus a single dose of equine chorionic gonadotrophin (eCG; 300 UI). The first FSH/eCG doses were administered 48 h after the sponge insertion, being repeated every 4 days to complete a total of four treatments in sheep and three in goats. Follicles in both ovaries were categorized according to their diameter and follicular fluid was aspirated under laparoscopic observation without a vacuum pump. In sheep, during a 12-day-period, a total of 347 follicles were aspirated with a recovery rate of 46.9%. In goats, during an 8-day-period, 219 follicles were aspirated with a recovery rate of 45.6%. In both species, there were no significant differences in the number of aspirated follicles, oocyte recovery rate and good quality oocyte recovery rate. However, in sheep the oocyte recovery rate was higher for large follicles, whereas in goats no such effect was detected. In summary, current results indicate that retrieval of oocytes can be maximized, without affecting oocyte quality, by repeating 'oneshot' FSH/eCG regimes and LOPUs at intervals as short as 4 days.
在绵羊和山羊身上确定了重复激素治疗和腹腔镜取卵（LOPU）对卵母细胞回收率和质量的可能影响。在6只成年美利奴绵羊和5只克里奥尔山羊中，通过前列腺素F（2α）类似物同步卵巢状态，并在48小时后插入阴道海绵。用单剂量的促卵泡素（FSH，60mg NIH-FSH-P1）加单剂量的马绒毛膜促性腺激素（eCG，300IU）刺激卵泡发育。首次FSH/eCG剂量在插入海绵后48小时给予，每4天重复一次，绵羊共进行4次治疗，山羊进行3次治疗。根据卵泡直径对双侧卵巢中的卵泡进行分类，并在腹腔镜观察下不用真空泵抽吸卵泡液。在绵羊中，在12天的时间里，共抽吸了347个卵泡，回收率为46.9%。在山羊中，在8天的时间里，抽吸了219个卵泡，回收率为45.6%。在这两个物种中，抽吸卵泡的数量、卵母细胞回收率和优质卵母细胞回收率均无显著差异。然而，在绵羊中，大卵泡的卵母细胞回收率较高，而在山羊中未检测到这种影响。总之，目前的结果表明，通过每4天重复一次“单次”FSH/eCG方案和LOPU，可以在不影响卵母细胞质量的情况下使卵母细胞的回收最大化。
