Svinhufvud M, Prellner K, Hermansson A, Schalén C
Department of Oto-Rhino-Laryngology University Hospital, Lund, Sweden.
Acta Otolaryngol. 1991;111(6):1083-9. doi: 10.3109/00016489109100760.
To elucidate immune mechanisms in otitis media, middle ear infection was induced in 12 rats by intratympanic inoculation of Streptococcus pneumoniae type 3, either the ipsilateral or the contralateral middle ear being re-challenged 4 weeks later. Otomicroscopy inspection confirmed the presence of acute otitis media (AOM) in all rats after the first challenge. After re-challenge, protection against AOM was noted both in the ipsilateral and contralateral ears. Serum antibody concentrations increased after the initial challenge, reaching a maximum at 4-7 days, but had decreased to pre-immune levels at re-challenge, after which no new increase was noted. Serum IgG-antibodies to pneumococcal type 3-polysaccharide were triggered following the initial induction of unilateral pneumococcal AOM, but mucosal immune mechanisms are argued to be a more probable explanation of the ipsilateral protection seen after re-challenge.
为阐明中耳炎的免疫机制，通过鼓室内接种3型肺炎链球菌在12只大鼠中诱发中耳感染，4周后对同侧或对侧中耳进行再次攻击。耳镜检查证实首次攻击后所有大鼠均存在急性中耳炎（AOM）。再次攻击后，同侧和对侧耳朵均出现了对AOM的保护作用。初次攻击后血清抗体浓度升高，在4-7天达到峰值，但在再次攻击时已降至免疫前水平，此后未观察到新的升高。单侧肺炎球菌性AOM初次诱发后可触发针对3型肺炎球菌多糖的血清IgG抗体，但黏膜免疫机制被认为更有可能解释再次攻击后同侧出现的保护作用。
