Solary E, Prud'homme J F, Gauville C, Magdelenat H, Calvo F
Pharmacology Laboratory, Hôpital Saint-Louis, Paris, France.
J Biol Regul Homeost Agents. 1991 Jul-Sep;5(3):98-106.
The effects of recombinant gamma interferon (IFN gamma) on proliferation, estrogen-receptor (ER) content, mRNA level and protein secretion of a breast cancer estrogen-induced protein pS2/BCEI were investigated in two human breast cancer cell lines, ZR75-1 and T47D. Both cell lines have estrogen and progesterone receptors and previously showed HLA class I and class II responses to IFN gamma (Int J Cancer 1990; 45: 1169). An antiproliferative effect of IFN gamma (1000-5000 U/ml) was observed in serum containing medium on ZR75-1 but not on T47D cells. Noninhibitory concentration of IFN gamma (100 U/ml) had sensitising antiproliferative effect with antiestrogens on ZR75-1 cells whereas IFN gamma did not modify the growth inhibition observed in T47D cells with antiestrogens. In serum-free, estradiol-free, phenol-red-free chemically defined medium (Cancer Res 1984; 44: 4553), IFN gamma abolished in ZR75-1 but not in T47D the 30% growth stimulation induced by estradiol. In ZR75-1 cells, IFN gamma induced a transitory 30-50% increase of ER content, as measured by ER-enzymoimmunoassay, at day 2 of culture, and reduced mRNA level and secretion of pS2/BCEI. In T47D cells, a 30-50% decrease of ER content was observed but only when cells were long term cultured (30 weeks) with IFN gamma. In this cell line, no transcription of pS2/BCEI was observed. Antiproliferative action of IFN gamma on ZR75-1 cells is associated with an inhibition of estradiol effects and a reduction of pS2/BCEI mRNA level and protein secretion.
在两种人乳腺癌细胞系ZR75-1和T47D中，研究了重组γ干扰素（IFNγ）对乳腺癌雌激素诱导蛋白pS2/BCEI的增殖、雌激素受体（ER）含量、mRNA水平及蛋白分泌的影响。这两种细胞系均有雌激素和孕激素受体，且先前已显示对IFNγ有HLA I类和II类反应（《国际癌症杂志》1990年；45: 1169）。在含血清培养基中，观察到IFNγ（1000 - 5000 U/ml）对ZR75-1细胞有抗增殖作用，但对T47D细胞无此作用。IFNγ的非抑制浓度（100 U/ml）与抗雌激素联合对ZR75-1细胞有增敏抗增殖作用，而IFNγ未改变T47D细胞中抗雌激素所观察到的生长抑制作用。在无血清、无雌二醇、无酚红的化学限定培养基中（《癌症研究》1984年；44: 4553），IFNγ消除了ZR75-1细胞中由雌二醇诱导的30%的生长刺激，但对T47D细胞无此作用。在ZR75-1细胞中，通过ER酶免疫测定法检测，IFNγ在培养第2天诱导ER含量短暂增加30 - 50%，并降低pS2/BCEI的mRNA水平及分泌。在T47D细胞中，仅当细胞用IFNγ长期培养（30周）时，观察到ER含量降低30 - 50%。在此细胞系中，未观察到pS2/BCEI的转录。IFNγ对ZR75-1细胞的抗增殖作用与抑制雌二醇作用以及降低pS2/BCEI的mRNA水平和蛋白分泌相关。
