Stoodley Robin, Wasan Kishor M, Bizzotto Dan
Advanced Materials and Process Engineering Laboratory, Department of Chemistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.
Langmuir. 2007 Aug 14;23(17):8718-25. doi: 10.1021/la7008573. Epub 2007 Jul 18.
Fluorescence excitation and emission spectra are reported for the polyene macrolide antifungal agent Amphotericin B formulated as micellar dispersion Fungizone (FZ) and its modified counterpart heat-treated Fungizone. The addition of sodium dodecyl sulfate or sodium deoxycholate surfactant to modulate the aggregation state of Amphotericin B confirms that the monomer and dimer states have different fluorescence spectra. Energy transfer from excited dimer to monomer is observed. Both FZ and heat-treated FZ (HTFZ) show expected S1 --> S0 fluorescence emission as well as anti-Kasha fluorescence emission from the S2 state. The excitation and S1 --> S0 emission spectra of HTFZ are similar to those of FZ, while the S2 --> S0 fluorescence differs in intensity between them. The variation in the rate constant for internal conversion from S2 to S1 as the surfactant concentration is increased differs for FZ and HTFZ; we propose that this may form a new basis for examining the super-aggregated character of AmB preparations. FZ and HTFZ have a similar stability to disaggregation by added sodium dodecyl sulfate surfactant. These findings provide the groundwork for future fluorescence characterization of FZ or HTFZ interactions with cell membranes.
报道了作为胶束分散体两性霉素B（Fungizone，FZ）及其经热处理的对应物（热处理的Fungizone，HTFZ）的多烯大环内酯类抗真菌剂的荧光激发和发射光谱。添加十二烷基硫酸钠或脱氧胆酸钠表面活性剂以调节两性霉素B的聚集状态，证实单体和二聚体状态具有不同的荧光光谱。观察到从激发的二聚体到单体的能量转移。FZ和热处理的FZ（HTFZ）均显示出预期的S1→S0荧光发射以及来自S2态的反卡莎荧光发射。HTFZ的激发光谱和S1→S0发射光谱与FZ相似，而它们之间的S2→S0荧光强度不同。随着表面活性剂浓度增加，从S2到S1的内转换速率常数的变化在FZ和HTFZ中有所不同；我们认为这可能为研究两性霉素B制剂的超聚集特性形成新的基础。FZ和HTFZ对添加的十二烷基硫酸钠表面活性剂的解聚具有相似的稳定性。这些发现为未来FZ或HTFZ与细胞膜相互作用的荧光表征奠定了基础。
