基于金纳米颗粒的氧化型谷胱甘肽向还原型谷胱甘肽还原过程的监测。

Gold nanoparticle-based monitoring of the reduction of oxidized to reduced glutathione.

作者信息

He Xiaorong, Zhong Zhifeng, Guo Yanbing, Lv Jing, Xu Jialiang, Zhu Mei, Li Yuliang, Liu Huibiao, Wang Shu, Zhu Yulan, Zhu Daoben

机构信息

Key Laboratory of Organic Solids, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beiijng 100080, P. R. China.

出版信息

Langmuir. 2007 Aug 14;23(17):8815-9. doi: 10.1021/la7011028. Epub 2007 Jul 18.

DOI:10.1021/la7011028

PMID:17637013

Abstract

We demonstrate the reduction of oxidized to reduced glutathione in the presence of glutathione reductase enzyme based on the modulation in photoluminescent quenching efficiency between the perylene bisimide chromophore TPPCA and gold nanoparticles (AuNPs). The TPPCA-AuNPs assembly shows a turn-on fluorescent signal in the presence of reduced glutathione, which provides a new concept to measure the balance of glutathione in a creature.

摘要

基于苝二酰亚胺发色团TPPCA与金纳米颗粒（AuNPs）之间光致发光猝灭效率的调节，我们证明了在谷胱甘肽还原酶存在的情况下，氧化型谷胱甘肽向还原型谷胱甘肽的转化。TPPCA-AuNPs组装体在还原型谷胱甘肽存在时显示开启荧光信号，这为测量生物体内谷胱甘肽的平衡提供了一个新概念。