White M T, Nandi S
J Natl Cancer Inst. 1976 Jan;56(1):65-73. doi: 10.1093/jnci/56.1.65.
Mitochondria isolated from spontaneous and transplanted mammary adenocarcinomas of two strains of mice were compared, by various biochemical criteria, to mitochondria from mammary glands of midpregnant or hormonally stimulated, cancer-free mice. The specific activities of several mitochondrial enzymes including cytochrome oxidase, alpha-glycerophosphate oxidase, and succinate dehydrogenase were twofold to threefold lower, whereas the activity of monoamine oxidase was two fold higher in tumor mitochondria. Malate dehydrogenase, adenylate kinase, and NADH oxidase showed similar levels of activity in tumor and midpregnant mammary gland mitochondria. In addition, mitochondrial polypeptide composition was analyzed by electrophoresis on sodium dodecyl sulfate-urea polyacrylamide gels. Midpregnant mammary gland and mammary tumor mitochondria were similar in polypeptide composition; however, several differences were observed. A high-molecular-weight polypeptide, present in mid-pregnant mammary gland mitochondria was absent from tumor mitochondria. Also, tumor mitochondria contained an additional high-molecular-weight polypeptide not found in the midpregnant mammary gland. There were numerous differences in the relative proportions of many polypeptides common to both tumor and midpregnant mammary gland mitochondria.
通过多种生化标准，对从两株小鼠的自发性和移植性乳腺腺癌中分离出的线粒体，与来自妊娠中期或激素刺激的无癌小鼠乳腺的线粒体进行了比较。几种线粒体酶的比活性，包括细胞色素氧化酶、α-磷酸甘油氧化酶和琥珀酸脱氢酶，降低了两倍到三倍，而单胺氧化酶的活性在肿瘤线粒体中则高两倍。苹果酸脱氢酶、腺苷酸激酶和NADH氧化酶在肿瘤和妊娠中期乳腺线粒体中的活性水平相似。此外，通过在十二烷基硫酸钠-尿素聚丙烯酰胺凝胶上进行电泳，分析了线粒体多肽组成。妊娠中期乳腺和乳腺肿瘤线粒体的多肽组成相似；然而，也观察到了一些差异。一种存在于妊娠中期乳腺线粒体中的高分子量多肽在肿瘤线粒体中不存在。此外，肿瘤线粒体含有一种在妊娠中期乳腺中未发现的额外的高分子量多肽。在肿瘤和妊娠中期乳腺线粒体共有的许多多肽的相对比例上存在许多差异。
