Dittrich Ralf, Beckmann Matthias W, Maake Caroline, Oppelt Patricia G, Mueller Andreas, Mueller Siegfried, Hoffmann Inge, Naumann Gert, Koelbl Heinz, Maltaris Theodoros
Department of Obstetrics and Gynecology, Erlangen University Hospital, Erlangen, Germany.
Neurourol Urodyn. 2007;26(7):1024-9. doi: 10.1002/nau.20443.
The objective of the study was to establish an experimental model for the extracorporeal perfusion of the pig detrusor. In order to validate this model we examined some biochemical parameters and determined the effect of carbachol on the contractility of perfused female pig bladders.
Twenty-six pig bladders were perfused with Krebs-Ringer bicarbonate-glucose buffer for a period up to 5 hr with the aim to preserve a viable organ, which would be responsive to contraction-inducing agents. The intravesical pressure of the bladder as well as the intraarterial pressure of the vesical arteries were recorded before and after administration of carbachol.
The perfusate pH, lactate, partial carbon dioxide tension, and the ATP content in the perfused tissue, all indicators of tissue ischemia or cell necrosis, showed a good preservation of the organ for up to 5 hr. Carbachol was able to induce contractions of the prefilled bladder with a complete draining of the bladder throughout the whole perfusion period.
We could demonstrate that this perfusion system was able to preserve the pig bladder in a functional condition, appropriate for the study of physiological questions.
本研究的目的是建立猪逼尿肌体外灌注的实验模型。为了验证该模型，我们检测了一些生化参数，并确定了卡巴胆碱对灌注的雌性猪膀胱收缩性的影响。
用 Krebs-Ringer 碳酸氢盐-葡萄糖缓冲液对 26 个猪膀胱进行长达 5 小时的灌注，目的是保存一个对收缩诱导剂有反应的有活力的器官。在给予卡巴胆碱之前和之后记录膀胱的膀胱内压以及膀胱动脉的动脉内压。
灌注液的 pH 值、乳酸、二氧化碳分压以及灌注组织中的 ATP 含量，这些组织缺血或细胞坏死的所有指标，都显示该器官在长达 5 小时内得到了良好的保存。卡巴胆碱能够诱导预充盈膀胱的收缩，并且在整个灌注期间膀胱完全排空。
我们能够证明这个灌注系统能够将猪膀胱维持在适合研究生理问题的功能状态。
