Ren Bao-Wei, Zhao Wei-Hong, Wang Jiang-Tao, Zou Jing-Zhong, Han Xiao-Tian, Wang Lu, Liu Ya-Lin
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Huan Jing Ke Xue. 2007 Apr;28(4):712-8.
The three-dimensional fluorescence of dissolved organic matter in the mesocosm with different nutrients enrichment experiments in Jiaozhou Bay was determined by using excitation-emission matrix spectrum. The result indicates that phytoplankton can produce protein-like and humic-like fluorescent matter. The protein-like fluorescence is composed of tyrosine-like fluorescence and tryptophan-like fluorescence. The main position of protein-like fluorescent peak is Ex(max)/Em(max) = 270 nm/290 - 310 nm. The fluorescent intensity of the peak located in Ex(max/ Em(max) = 270 - 290 nm/320 - 350 nm is less. The centers of humic-like peaks disperse at Ex(max)/Em(max) = 250 - 260 nm/380 - 480 nm (Peak A), Ex(max)/Em(max) = 310 - 320 nm/380 - 420 nm(Peak C) and Ex(max)/Em(max) = 330 - 350 nm/420 - 480 nm(Peak M) in which peak A is the main peak. The fluorescent intensity of tyrosine-like matter is stronger than the intensity of humic-like matter. When the amount of phytoplankton decreased, the fluorescent intensity of tyrosine-like matter has negative relativity with the chlorophyll-a concentration. Tyrosine-like matter and tryptophan-like matter have similar origin. Dinoflagellate can produce more protein-like fluorescent matter than diatom. The composition ratios of humic-like mixture are different in different environment. And it has a small A/C value in dinoflagellate environment compared to diatom environment.
利用激发发射矩阵光谱法测定了胶州湾不同营养盐加富实验围隔中溶解有机物的三维荧光光谱。结果表明，浮游植物能够产生类蛋白和类腐殖质荧光物质。类蛋白荧光由类酪氨酸荧光和类色氨酸荧光组成。类蛋白荧光峰的主要位置为Ex(max)/Em(max)=270nm/290 - 310nm。位于Ex(max)/Em(max)=270 - 290nm/320 - 350nm处的荧光峰强度较小。类腐殖质荧光峰中心分布在Ex(max)/Em(max)=250 - 260nm/380 - 480nm（峰A）、Ex(max)/Em(max)=310 - 320nm/380 - 420nm（峰C）和Ex(max)/Em(max)=330 - 350nm/420 - 480nm（峰M）处，其中峰A为主峰。类酪氨酸物质的荧光强度强于类腐殖质物质。当浮游植物数量减少时，类酪氨酸物质的荧光强度与叶绿素a浓度呈负相关。类酪氨酸物质和类色氨酸物质来源相似。甲藻比硅藻能产生更多的类蛋白荧光物质。不同环境中类腐殖质混合物的组成比例不同。与硅藻环境相比，甲藻环境中的A/C值较小。
