Lei La-Mei, Han Bo-Ping, Song Li-Rong
Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China.
Huan Jing Ke Xue. 2007 Apr;28(4):872-5.
A direct-competitive time-resolved fluorescence immunoassay (TRFIA) for microcystin detection was established, which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG. The optimal dilution of europium labeled MCLR-BSA conjugant is 1/50 and most appropriate titration of anti-microcystin-LR (MCLR) monoclonal antibodies is 100 ng/mL. The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05 ng/mL to 10 ng/mL. The detection limit of the method is 0.02 ng/mL for MCLR, recoveries for microcystin (> 94%) in quantitative range is satisfactory.
建立了一种基于铕标记的微囊藻毒素-LR-牛血清白蛋白（MCLR-BSA）偶联物和包被有抗小鼠IgG的微孔板的直接竞争时间分辨荧光免疫分析法（TRFIA）用于微囊藻毒素检测。铕标记的MCLR-BSA偶联物的最佳稀释度为1/50，抗微囊藻毒素-LR（MCLR）单克隆抗体的最适滴定度为100 ng/mL。最佳条件下的标准曲线表明，定量范围约为0.05 ng/mL至10 ng/mL。该方法对MCLR的检测限为0.02 ng/mL，定量范围内微囊藻毒素的回收率（>94%）令人满意。
