Yu Zhi-Hua, Wang Dong-Jian, Lin Shui-Miao
Shanghai Geriatrics Institute of Traditional Chinese Medicine, Shanghai 200031, China.
Zhongguo Zhong Yao Za Zhi. 2007 May;32(9):839-42.
To research the mechanism of TX0201 abstracted from heart-regulating formula in the treatment of rat with Alzheimer's disease (AD).
The rat models of AD were induced by A beta(1-40) injected into the bilateral amygdale. All rats were divided into five groups at random, namely control group, model group, heart-regulating formula group, TX0201 group and aricept group. The rats were intragastrically treated with different solution respectively for 20 days. The effect of TX0201 on spatial learning and memory ability of these rat models was investigated with the method of Morris water maze. By using the method of RT-PCR, the expression of apoptosis associated genes (such as Bcl-2 and Bax) in cerebral cortex and hippocampus of rats were examined.
Heart-regulating formula, TX0201 and aricept could significantly improve the spatial learning and memory ability of rats. Heart-regulating formula could comprehensively redress the abnormal expression of Bax mRNA and Bcl-2 mRNA in cerebral cortex and hippocampus. TX0201 could rectify the abnormal expression of Bax mRNA in cerebral cortex and hippocampus. Aricept could regulate the abnormal expression of Bax mRNA in cerebral cortex.
TX0201 could ameliorate the learning and memory ability of these model rats by alleviating neuron apoptosis by means of regulating the abnormal expression of Bax mRNA in brain.
研究从调心方中提取的TX0201对阿尔茨海默病（AD）大鼠的治疗机制。
通过向双侧杏仁核注射Aβ(1-40)诱导建立AD大鼠模型。将所有大鼠随机分为五组，即对照组、模型组、调心方组、TX0201组和多奈哌齐组。分别用不同溶液对大鼠进行灌胃处理20天。采用Morris水迷宫法研究TX0201对这些大鼠模型空间学习和记忆能力的影响。运用RT-PCR法检测大鼠大脑皮层和海马中凋亡相关基因（如Bcl-2和Bax）的表达。
调心方、TX0201和多奈哌齐均可显著提高大鼠的空间学习和记忆能力。调心方可全面纠正大脑皮层和海马中Bax mRNA和Bcl-2 mRNA的异常表达。TX0201可纠正大脑皮层和海马中Bax mRNA的异常表达。多奈哌齐可调节大脑皮层中Bax mRNA的异常表达。
TX0201可通过调节脑中Bax mRNA的异常表达减轻神经元凋亡，从而改善模型大鼠的学习和记忆能力。
Zotero 插件