Wiles Siouxsie, Crepin Valerie F, Childs Gary, Frankel Gad, Kerton Angela
Division of Cell and Molecular Biology, Natural Sciences, Imperial College London, London SW7 2AZ, UK.
Lab Anim. 2007 Jul;41(3):321-8. doi: 10.1258/002367707781282785.
Dosing of experimental animals and the removal of blood are two of the most frequent procedures performed in biomedical research using live animals. Despite the apparently simple nature of these procedures, they can, if not correctly carried out, have significant effects on the welfare of the animals and the scientific value of the results. There are several methods by which research staff may obtain training in the administration of substances. These include practical demonstrations during teaching courses; observation of techniques; videos and educational computer programs and practising on recently killed animal cadavers or plastic animal models. Each method has its own advantages and disadvantages. A common factor encountered during training is the difficulty in assessing competency. This paper reports a pilot study on the use of bioluminescent imaging technology to assess competency in the administration of substances to rodents. Bioluminescence was rapidly detected after dosing of animals with a bioluminescent substance. However, living animals were required for a signal to be generated. The data presented suggest that this technology is ideal for use as a teaching aid and may also prove valuable in assessing the effectiveness of 'complex' and novel administration routes in 'realtime'.
对实验动物给药和采血是生物医学研究中使用活体动物时最常进行的两项操作。尽管这些操作表面上性质简单，但如果执行不当，可能会对动物福利和结果的科学价值产生重大影响。研究人员可以通过多种方法获得物质给药方面的培训。这些方法包括教学课程中的实际演示；技术观察；视频和教育计算机程序以及在刚处死的动物尸体或塑料动物模型上进行练习。每种方法都有其自身的优缺点。培训过程中常见的一个因素是难以评估能力。本文报告了一项关于使用生物发光成像技术评估对啮齿动物给药能力的试点研究。给动物注射生物发光物质后能迅速检测到生物发光。然而，需要活体动物才能产生信号。所呈现的数据表明，这项技术非常适合用作教学辅助工具，并且在“实时”评估“复杂”和新颖给药途径的有效性方面可能也很有价值。
