Kolkert Joe L P, 't Hart Nils A, van Dijk Antony, Ottens Petra J, Ploeg Rutger J, Leuvenink Henri G D
Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Lab Anim. 2007 Jul;41(3):363-71. doi: 10.1258/002367707781282848.
Organs used for transplantation are usually derived from heart-beating brain dead donors. However, brain death is known to have negative effects on donor organ quality, previously studied using a difficult to control sudden onset experimental model. We have now developed a reproducible gradual onset brain death model in rats without requiring inotropic support. Fisher inbred rats weighing 260-300 g were used. Brain death was induced by a gradual inflation of a subdurally placed balloon catheter. During induction and the period following brain death, the animals were mechanically ventilated and blood pressure was continuously monitored. The blood pressure registration showed a characteristic pattern during brain death induction, in which a decrease in blood pressure, a hypotensive period in which the Cushing response occurred, and a sharp peak were consistent findings. After brain death was induced, blood pressure was maintained at normotensive levels up to 4 h. After the experiments, neuropathological evaluation of the brain located haemorrhagic cerebral parenchyma, and immunocytochemistry of liver tissue revealed a significant influx of polymorph nuclear cells, as was previously observed as well. This improved model allows the study of brain death on donor organ quality without the use of inotropic support.
用于移植的器官通常取自心跳脑死亡供体。然而，已知脑死亡会对供体器官质量产生负面影响，此前使用难以控制的突发实验模型进行过相关研究。我们现已开发出一种无需使用正性肌力药物支持的、可重复的大鼠渐进性脑死亡模型。使用体重260 - 300克的近交系Fisher大鼠。通过在硬膜下放置球囊导管逐渐充气诱导脑死亡。在诱导过程及脑死亡后的时间段内，对动物进行机械通气并持续监测血压。血压记录显示在脑死亡诱导过程中有一个特征性模式，即血压下降、出现库欣反应的低血压期以及一个尖峰是一致的发现。诱导脑死亡后，血压在长达4小时内维持在正常血压水平。实验结束后，对大脑进行神经病理学评估发现脑实质出血，对肝组织进行免疫细胞化学检测显示多形核细胞大量涌入，这与之前观察到的情况相同。这个改进后的模型允许在不使用正性肌力药物支持的情况下研究脑死亡对供体器官质量的影响。
