Ludwig Boltzmann Cluster for Cardiovascular Research, C/O Core Unit for Biomedical Research, Medical University of Vienna, Vienna General Hospital, Währinger Gürtel 18-20, 1090 Vienna, Austria.
J Neurosci Methods. 2010 Sep 30;192(1):70-4. doi: 10.1016/j.jneumeth.2010.07.019. Epub 2010 Jul 23.
Experimental animal models of brain death increasing intracranial pressure (ICP) by inflating an intracranial placed balloon-catheter are well established and used in transplant-associated studies. Our aim was to develop an experimental mouse model of brain death (BD) and to compare explosive and gradual brain death induction under ICP monitoring. We therefore induced BD in female OF-1 mice by injecting 40 microl saline every 5 min into an intracranial placed balloon increasing ICP rapidly [BD ex, n=7], or gradually [BD grad, n=7] with 20 microl volume every 5 min under electroencephalogram (EEG) and ICP monitoring until BD occurred. The major criterion for BD was a flat-line-EEG, confirmed by cessation of spontaneous respiration and maximally dilated and fixed pupils. ICP, central activity and heart rate were continuously monitored during the entire 6h follow-up. In sham-operated controls [control, n=7] a burr hole was drilled but no balloon inserted. The BD groups showed equal ICP levels at the time of BD. Both groups had increased heart rates (HR) 15 min after BD, HR decreased to 402+/-29.39 bpm (beats per minute) [BD ex] and 409.33+/-26.46 bpm [BD grad] (n.s. vs. control) by 30 min after the inflation of the balloon, but only BD ex showed a significant decrease in HR compared to control, progressively decreasing thereafter. On the basis of these results, we conclude that the mouse model of brain death can be performed in a standardized, reproducible and successful way.
通过向颅内放置的球囊导管充气来升高颅内压 (ICP) 的实验性脑死亡动物模型已经建立并广泛应用于移植相关研究。我们的目的是建立一种实验性脑死亡 (BD) 小鼠模型，并比较在 ICP 监测下进行的爆发性和逐渐性脑死亡诱导。因此，我们通过向颅内放置的球囊内每 5 分钟注射 40 微升盐水来诱导 OF-1 雌性小鼠的 BD [BD ex，n=7]，或者在 EEG 和 ICP 监测下以 20 微升体积每 5 分钟逐渐增加 [BD grad，n=7]，直到发生 BD。BD 的主要标准是 EEG 呈平线，通过停止自主呼吸和最大程度扩张和固定的瞳孔来确认。在整个 6 小时的随访过程中，连续监测 ICP、中心活动和心率。在假手术对照 [control，n=7] 中，钻一个骨孔，但不插入球囊。BD 组在发生 BD 时具有相同的 ICP 水平。两组在 BD 后 15 分钟均出现心率 (HR) 增加，HR 在 30 分钟后降至 402+/-29.39 bpm（每分钟心跳次数）[BD ex]和 409.33+/-26.46 bpm [BD grad]（与对照相比无统计学意义），但只有 BD ex 与对照相比 HR 显著下降，此后逐渐下降。基于这些结果，我们得出结论，脑死亡小鼠模型可以以标准化、可重复和成功的方式进行。
