不同细胞培养系统中抗病毒药物对猫免疫缺陷病毒活性的比较评估。

Comparative evaluation of the activity of antivirals towards feline immunodeficiency virus in different cell culture systems.

作者信息

van der Meer F J U M, Schuurman N M P, Balzarini J, Egberink H F

机构信息

Department of Infectious Diseases and Immunology, Division of Virology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.

出版信息

Antiviral Res. 2007 Nov;76(2):198-201. doi: 10.1016/j.antiviral.2007.06.003. Epub 2007 Jul 2.

DOI:10.1016/j.antiviral.2007.06.003

PMID:17640744

Abstract

Influences of the cell system on observed EC(50) values of different agents against feline immunodeficiency virus (FIV) were assessed. The activity of various nucleoside reverse transcriptase inhibitors (NRTI) against a lymphotropic FIV strain was evaluated using monocultured thymocytes and a DC-thymocyte coculture. In the second set of experiments activity of carbohydrate binding agents (CBA) towards FIV strains derived from different cell lines (e.g. Crandall feline kidney cells (CRFK) and thymocytes) was compared. We examined three different FIV-based antiviral evaluation systems and obtained marked differences in EC(50) values, especially for CBA entry inhibitors. Our study confirms and extends earlier observed differences between cell systems used for the evaluation of the activity of antivirals towards FIV.

摘要

评估了细胞系统对不同药物抗猫免疫缺陷病毒（FIV）观察到的半数有效浓度（EC50）值的影响。使用单培养的胸腺细胞和树突状细胞 - 胸腺细胞共培养物评估了各种核苷类逆转录酶抑制剂（NRTI）对嗜淋巴细胞性FIV毒株的活性。在第二组实验中，比较了碳水化合物结合剂（CBA）对源自不同细胞系（如克兰德尔猫肾细胞（CRFK）和胸腺细胞）的FIV毒株的活性。我们研究了三种不同的基于FIV的抗病毒评估系统，并在EC50值上获得了显著差异，尤其是对于CBA进入抑制剂。我们的研究证实并扩展了早期观察到的用于评估抗病毒药物对FIV活性的细胞系统之间的差异。