Tanabe T, Yamamoto J K
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
J Interferon Cytokine Res. 2001 Dec;21(12):1039-46. doi: 10.1089/107999001317205169.
The antiviral activity of recombinant feline interferon-gamma (rFeIFN-gamma) against feline immunodeficiency virus (FIV) was investigated. A persistently FIV(Bang)-infected feline T cell line (FeT-J/Bang) was treated with either rFeIFN-omega, rFeIFN-gamma, or recombinant human IFN-alpha2 (rHuIFN-alpha2), and the culture fluids were tested for antiviral activity by reverse transcriptase (RT) assay. FeT-J/Bang cell cultures treated with rFeIFN-omega showed dose-dependent inhibition of RT activity. In contrast, rFeIFN-gamma treatment had no antiviral effect on FIV replication but instead caused a statistically significant enhancement on day 9 of culture. Antiviral activity of rFeIFN-gamma was also tested on feline peripheral blood mononuclear cells (PBMC). PBMC cultures were inoculated with FIV(Bang) and simultaneously treated with either rFeIFN-omega, rFeIFN-gamma, or rHuIFN-alpha2. FeIFN-gamma had no effect on FIV replication, unlike the rFeIFN-omega and rHuIFN-alpha2, which had strong anti-FIV effects. In another study, rFeIFN-gamma treatment was initiated 3 days before FIV(Bang) infection, the day of FIV(Bang) infection, or 3 days post-FIV(Bang) infection and then tested for antiviral activity. The time of initiating rFeIFN-gamma treatment had no effect on the antiviral activity. Hence, these results suggest that unlike rHuIFN-alpha2 and rFeIFN-omega, rFeIFN-gamma has no inhibitory effect on FIV replication in PBMC but causes a slight enhancement in a feline T cell line.
研究了重组猫干扰素-γ（rFeIFN-γ）对猫免疫缺陷病毒（FIV）的抗病毒活性。用rFeIFN-ω、rFeIFN-γ或重组人干扰素-α2（rHuIFN-α2）处理持续感染FIV（Bang）的猫T细胞系（FeT-J/Bang），并通过逆转录酶（RT）测定法检测培养液的抗病毒活性。用rFeIFN-ω处理的FeT-J/Bang细胞培养物显示出RT活性的剂量依赖性抑制。相反，rFeIFN-γ处理对FIV复制没有抗病毒作用，反而在培养第9天引起统计学上的显著增强。还在猫外周血单个核细胞（PBMC）上测试了rFeIFN-γ的抗病毒活性。PBMC培养物接种FIV（Bang），并同时用rFeIFN-ω、rFeIFN-γ或rHuIFN-α2处理。与具有强抗FIV作用的rFeIFN-ω和rHuIFN-α2不同，FeIFN-γ对FIV复制没有影响。在另一项研究中，在FIV（Bang）感染前3天、FIV（Bang）感染当天或FIV（Bang）感染后3天开始rFeIFN-γ处理，然后测试抗病毒活性。开始rFeIFN-γ处理的时间对抗病毒活性没有影响。因此，这些结果表明，与rHuIFN-α2和rFeIFN-ω不同，rFeIFN-γ对PBMC中的FIV复制没有抑制作用，但在猫T细胞系中会引起轻微增强。
