Atomi Y, Yamada S, Nishida T
Department of Sports Sciences, College of Arts & Sciences, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1323-30. doi: 10.1016/0006-291x(91)92083-v.
alpha B-Crystallin specifically decreases in atrophied rat soleus muscle with hindlimb suspension (HS). alpha B-Crystallin cDNA was cloned from rat heart cDNA library using oligonucleotide probe, and its complete coding and partial non-coding regions were sequenced. Northern blot analysis revealed that alpha B-crystallin mRNA in slow muscle decreases at 36 hour after HS but recovered at 24 hour after HS stopped. Denervation decreased the expression of alpha B-crystallin mRNA in slow muscle but increased it in fast muscles, which hardly expressed in normal condition. Passive tension increased the expression of alpha B-crystallin mRNA in both muscle types. Based upon these Northern blot analysis of alpha B-crystallin, nerve innervation and external load on muscle are essential regulatory factors on the expression of the mRNA of alpha B-crystallin in rat skeletal muscle.
αB-晶状体蛋白在大鼠后肢悬吊（HS）导致的萎缩比目鱼肌中特异性减少。使用寡核苷酸探针从大鼠心脏cDNA文库中克隆αB-晶状体蛋白cDNA，并对其完整编码区和部分非编码区进行测序。Northern印迹分析显示，慢肌中的αB-晶状体蛋白mRNA在HS后36小时减少，但在HS停止后24小时恢复。去神经支配降低了慢肌中αB-晶状体蛋白mRNA的表达，但增加了快肌中αB-晶状体蛋白mRNA的表达，而快肌在正常情况下几乎不表达。被动张力增加了两种肌肉类型中αB-晶状体蛋白mRNA的表达。基于对αB-晶状体蛋白的这些Northern印迹分析，神经支配和肌肉的外部负荷是大鼠骨骼肌中αB-晶状体蛋白mRNA表达的重要调节因子。
