结直肠癌中p53和Ki67免疫染色与bcl-2表达及经典预后因素之间的相关性。

The correlation between the immunostains for p53 and Ki67 with bcl-2 expression and classical prognostic factors in colorectal carcinomas.

作者信息

Gurzu Simona, Jung J, Mezei T, Pávai Z

机构信息

Department of Pathology, University of Medicine and Pharmacy of Târgu Mureş, 38 Gheorghe Marinescu Street, Târgu Mureş, Romania.

出版信息

Rom J Morphol Embryol. 2007;48(2):95-9.

PMID:17641794

Abstract

UNLABELLED

The prognostic role of p53 and Ki67 in colorectal carcinomas (CRC) is very controversial in the literature. In our study, we tried to find if their immunostains are correlated with bcl-2 expression or other classical prognostic factors (sex, age, localization and size of tumor, the grade and staging of tumor). We studied 507 cases with CRC and chose 38 cases in which we realized these correlations. Fourteen cases were mucinous CRC, the other 24 cases being non-mucinous CRC (six well differentiated, 13 moderate and five poorly differentiated). For statistical analysis, we used the Statistical Program Graph Pad In Stat 3-Trial Version. We considered the significant association when p<0.05, with 95% confidence interval.

RESULTS

The median value was 75% for p53 expression, respectively 35% for Ki67 expression. Bcl-2 was positive in 47% of cases but not correlated with p53 or Ki67. We found a significantly statistical decrease p53 immunostain with grade of tumor (70% in well differentiated, respectively 40% in poorly differentiated CRC) and increase of Ki67 median expression (25% in well differentiated, respectively 60% in poorly differentiated CRC). Ki67 was correlated with age of patients, lymph node involvement, being more expressed in N2 (80%) than in N0 (22.5%) and with Dukes MAC staging (25% in B1, 60% in C2). P53 was correlated with age of patients and pT component, after pTNM staging (75% in pT2, 40% in pT4). P53 was not correlated with Ki67.

CONCLUSION

The CCR prognostic is not determined only by proliferative capacity of tumoral cells.

摘要

未标记

p53和Ki67在结直肠癌（CRC）中的预后作用在文献中存在很大争议。在我们的研究中，我们试图找出它们的免疫染色是否与bcl-2表达或其他经典预后因素（性别、年龄、肿瘤定位和大小、肿瘤分级和分期）相关。我们研究了507例CRC患者，并选择了38例实现这些相关性的病例。14例为黏液性CRC，其他24例为非黏液性CRC（6例高分化，13例中分化，5例低分化）。为了进行统计分析，我们使用了统计程序Graph Pad In Stat 3试用版。当p<0.05且置信区间为95%时，我们认为存在显著关联。

结果

p53表达的中位数为75%，Ki67表达的中位数为35%。bcl-2在47%的病例中呈阳性，但与p53或Ki67无关。我们发现p53免疫染色随肿瘤分级有显著统计学下降（高分化CRC中为70%，低分化CRC中为40%），Ki67中位数表达增加（高分化CRC中为25%，低分化CRC中为60%）。Ki67与患者年龄、淋巴结受累相关，在N2中表达更高（80%），而在N0中为22.5%，并且与Dukes MAC分期相关（B1中为25%，C2中为60%）。P53与患者年龄和pT成分相关，在pTNM分期后（pT2中为75%，pT4中为40%）。P53与Ki67无关。