Zhang Juan, Zhu Guijin, Wang Xinrong, Xu Bei, Hu Linli
Reproductive Medical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2007 Jun;27(3):311-4. doi: 10.1007/s11596-007-0324-6.
The relationship between apoptosis of granulosa cells and follicle development arrest in polycystic ovarian syndrome (PCOS) rats, and the contribution of tumor necrosis factor related apoptosis inducing ligand (TRAIL) in apoptosis of granulosa cells were explored. By using sodium prasterone sulfate rat PCOS model was induced. The apoptosis of granulosa cells in ovaries of rats was observed by TdT-mediated dUTP-biotin nick end-labeling (TUNEL), and the expression of TRAIL protein and mRNA in granulosa cells was detected by using immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) respectively. The apoptotic rate and the expression of protein TRAIL in granulosa cells were significantly higher in antral follicles from the PCOS rats than in those from the control rats (P<0.01, P<0.05). There was no significant difference in apoptotic rate and the expression of TRAIL protein in granulosa cells of preantral follicles between the PCOS rats and the control rats (P>0.05). No apoptosis and the expression of TRAIL protein in granulosa cells of primordial follicles were found in the two groups. The expression of TRAIL mRNA was significantly stronger in granulosa cells from the PCOS rats than in those from the control rats (P<0.01). It was suggested that the apoptotic rate in granulosa cells was significantly higher in antral follicle from the PCOS rats than in those from the control rats. TRAIL played a role in regulating the apoptosis of granulosa cells in PCOS rats.
探讨多囊卵巢综合征（PCOS）大鼠颗粒细胞凋亡与卵泡发育停滞之间的关系，以及肿瘤坏死因子相关凋亡诱导配体（TRAIL）在颗粒细胞凋亡中的作用。采用硫酸普拉睾酮钠诱导大鼠PCOS模型。通过TdT介导的dUTP生物素缺口末端标记法（TUNEL）观察大鼠卵巢颗粒细胞凋亡情况，分别采用免疫组化染色和逆转录聚合酶链反应（RT-PCR）检测颗粒细胞中TRAIL蛋白和mRNA的表达。PCOS大鼠窦状卵泡颗粒细胞的凋亡率和TRAIL蛋白表达显著高于对照组大鼠（P<0.01，P<0.05）。PCOS大鼠与对照组大鼠窦前卵泡颗粒细胞的凋亡率和TRAIL蛋白表达无显著差异（P>0.05）。两组原始卵泡颗粒细胞均未发现凋亡及TRAIL蛋白表达。PCOS大鼠颗粒细胞中TRAIL mRNA的表达显著强于对照组大鼠（P<0.01）。提示PCOS大鼠窦状卵泡颗粒细胞的凋亡率显著高于对照组大鼠。TRAIL在PCOS大鼠颗粒细胞凋亡调控中发挥作用。
