Rastawicki Waldemar
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 2006;58(4):303-19.
The antibodies against the somatic antigens of Y. enterocolitica O3, O8, O9, O5,27,Y. pseudotuberculosis I, and released proteins Yop were detected using the ELISA in 1634 serum samples and 84 synovial fluids collected from 1290 persons suspected for yersiniosis, as well as 200 serum samples from healthy individuals (blood donors). The presence of antibody in diagnostically significant titres for somatic antigens of Yersinia were detected by ELISA in 20.5% and 50.6%, antibodies for released proteins Yop in 11.5% and 28.4% respectively of blood donors and patients suspected for yersiniosis. The antibody against the O3 antigen of Y. enterocolitica was the most frequently detected antibody while the most infrequent was the antibody for the antigen from the 08 serologic group. The results of the study showed that the humoral response picture to Yersinia antigens in the course of yersiniosis in humans is dependent on the age and sex of the patient, duration of the infection, and clinical manifestations. Most frequently the elevated antibody levels were detected among patients with erythema nodosum and patients with gastrointestinal symptoms. The frequency of occurrence of antibodies for most antigens of Yersinia, together with age increased reaching its peak, on the average, among individuals aged 21 - 40 years. Analysis of individual cases showed that by the end of the first week of infection, elevated levels of antibodies for somatic antigens of Yersinia are evident. On the other hand, antibodies for released proteins Yop as a matter of rule appear in the second week from the onset of clinical symptoms. Within this early phase of infection immunoglobulins of the A and M classes dominate reaching their highest level in the second to third week of the infection. In majority of the individuals studied antibodies of the IgG class reached their highest level much later in relation to those of the IgA and IgM classes. Significant differences were found in IgA antibody detection among individuals with clinical manifestations of stomachaches and arthritis. Nevertheless, among individuals with clinical symptoms of stomachaches, these immunoglobulins as a matter of principle disappear with a period of 2-3 months from the onset of clinical symptoms. In individuals with arthritis however the aforementioned immunoglobulins maintained at considerable levels even after a year. In joint-fluid samples obtained from patients with arthritis antibodies for Yersinia antigens were detected in similar levels just as obtained simultaneously serum from those individuals.
采用酶联免疫吸附测定法(ELISA),对1634份血清样本和84份滑液进行检测,这些样本分别采自1290名疑似耶尔森氏菌病患者以及200名健康个体(献血者),以检测抗小肠结肠炎耶尔森氏菌O3、O8、O9、O5,27、假结核耶尔森氏菌I菌体抗原的抗体以及Yop分泌蛋白的抗体。ELISA检测结果显示,献血者和疑似耶尔森氏菌病患者中,针对耶尔森氏菌菌体抗原,具有诊断意义滴度的抗体检出率分别为20.5%和50.6%;针对Yop分泌蛋白的抗体检出率分别为11.5%和28.4%。抗小肠结肠炎耶尔森氏菌O3抗原的抗体是最常检测到的抗体,而08血清群抗原的抗体则是最不常检测到的。研究结果表明,人类耶尔森氏菌病病程中对耶尔森氏菌抗原的体液免疫反应情况取决于患者的年龄、性别、感染持续时间及临床表现。结节性红斑患者和有胃肠道症状的患者中,抗体水平升高最为常见。耶尔森氏菌大多数抗原抗体的出现频率随年龄增加而升高,平均在21 - 40岁个体中达到峰值。对个别病例的分析表明,感染第一周结束时,耶尔森氏菌菌体抗原抗体水平明显升高。另一方面,Yop分泌蛋白抗体通常在临床症状出现后的第二周出现。在感染的早期阶段,A类和M类免疫球蛋白占主导,在感染的第二至第三周达到最高水平。在大多数研究个体中,IgG类抗体水平达到最高值的时间比IgA和IgM类抗体晚得多。在有胃痛和关节炎临床表现的个体中,IgA抗体检测存在显著差异。然而,在有胃痛临床症状的个体中,这些免疫球蛋白通常在临床症状出现后的2 - 3个月内消失。而在患有关节炎的个体中,上述免疫球蛋白即使在一年后仍维持在相当高的水平。在患有关节炎患者的关节液样本中,检测到的耶尔森氏菌抗原抗体水平与同时采集的这些个体的血清样本中的水平相似。