Telli-Okur Müjgan, Eken-Saraçoğlu Nurdan
Department of Chemical Engineering, Faculty of Architecture and Engineering, Gazi University, Maltepe 06570, Ankara, Turkey.
Bioresour Technol. 2008 May;99(7):2162-9. doi: 10.1016/j.biortech.2007.05.036. Epub 2007 Jul 20.
Ethanol production from sunflower seed hull hydrolysate was evaluated using Pichia stipitis NRRL Y-7124. The hydrolysate prepared with 0.7 M H2SO4 at 90 degrees C was fermented as substrate in shaking bath experiments at 30 degrees C. In a group of experiments, the influence of various detoxification methods on the fermentability of hydrolysate was investigated at pH 6. Even though the ability of all employed pretreatments to enhance fermentation performance was close, the sequential application of overliming with sodium sulfite addition was the best detoxification method. Additional experiments were performed with detoxified hydrolysate to investigate the effect of shaking rate (70-130 rpm) and initial pH (5.5-7) on the fermentation. The highest ethanol level 11 gL(-1) was achieved at initial pH of 6 and 100 rpm shaking rate from a hydrolysate containing 48 gL(-1) total reducing sugar. The corresponding alcohol yield and volumetric productivity were 0.32 gg(-1) and 0.065 gL(-1)h(-1).
使用树干毕赤酵母NRRL Y - 7124评估了从向日葵籽壳水解物中生产乙醇的情况。在90℃下用0.7 M硫酸制备的水解物在30℃的摇瓶实验中作为底物进行发酵。在一组实验中，在pH 6的条件下研究了各种解毒方法对水解物发酵能力的影响。尽管所有采用的预处理提高发酵性能的能力相近，但先进行过石灰处理再添加亚硫酸钠是最佳的解毒方法。用解毒后的水解物进行了额外实验，以研究摇床转速（70 - 130转/分钟）和初始pH值（5.5 - 7）对发酵的影响。从含有48 g/L总还原糖的水解物中，在初始pH值为6和摇床转速为100转/分钟时获得了最高乙醇水平11 g/L。相应的酒精产率和体积生产率分别为0.32 g/g和0.065 g/(L·h)。
