纳豆枯草芽孢杆菌T-2产生的环状脂肽诱导人白血病K562细胞凋亡
Induction of apoptosis in human leukemia K562 cells by cyclic lipopeptide from Bacillus subtilis natto T-2.
作者信息
Wang C L, Ng T B, Yuan F, Liu Z K, Liu F
机构信息
Department of Microbiology, College of Life Science, Nankai University, Tianjin, China.
出版信息
Peptides. 2007 Jul;28(7):1344-50. doi: 10.1016/j.peptides.2007.06.014. Epub 2007 Jun 20.
A new cyclic lipopeptide (CLP) purified from Bacillus subtilis natto T-2 dose dependently inhibited growth in human leukemia K562 cells. The results of fluorescent staining indicated that CLP brought about apoptosis in K562 cells. Flow cytometric analysis also demonstrated that CLP caused dose-dependent apoptosis of K562 cells through cell arrest at G1 phase. Western blotting revealed that CLP-induced apoptosis in K562 cells was associated with caspase-3 and poly(ADP-ribose)polymerase (PARP) protein. It is estimated that CLP inhibited proliferation in K562 cells by inducing apoptosis.
从纳豆芽孢杆菌T-2中纯化得到的一种新型环脂肽（CLP）对人白血病K562细胞的生长具有剂量依赖性抑制作用。荧光染色结果表明，CLP可诱导K562细胞凋亡。流式细胞术分析也表明，CLP通过使K562细胞停滞于G1期而导致其剂量依赖性凋亡。蛋白质免疫印迹法显示，CLP诱导K562细胞凋亡与半胱天冬酶-3和聚（ADP-核糖）聚合酶（PARP）蛋白有关。据估计，CLP通过诱导凋亡来抑制K562细胞的增殖。
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