Cao Xiao-Hong, Liao Zhen-Yu, Wang Chun-Ling, Cai Ping, Yang Wen-Yan, Lu Mei-Fang, Huang Guo-Wei
School of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
Biotechnol Appl Biochem. 2009 Feb;52(Pt 2):97-106. doi: 10.1042/BA20070227.
An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.
从纳豆芽孢杆菌TK-1中纯化得到的一种抗肿瘤脂肽生物表面活性剂能够以剂量和时间依赖性方式抑制MCF-7人乳腺癌细胞的增殖。用脂肽处理的MCF-7细胞与未处理的对照相比，乳酸脱氢酶释放活性无显著差异。脂肽在MCF-7细胞中的抗肿瘤活性与通过典型形态学变化和细胞生长阶段分布中的亚G(1)峰确定的细胞凋亡有关。细胞周期停滞在G(2)/M期。此外，半胱天冬酶活性测定表明，脂肽诱导的MCF-7细胞凋亡与半胱天冬酶3有关。
