Graack H R, Grohmann L, Kitakawa M
Ruhr-Universität Bochum, Fakultät für Chemie, Lehrstuhl für Biochemie, Germany.
Biochimie. 1991 Jun;73(6):837-44. doi: 10.1016/0300-9084(91)90063-7.
Using synthetic oligonucleotides deduced from the N-terminal amino acid sequence of purified mitoribosomal protein (mt r-protein) YmL27, the corresponding nuclear gene MRP-L27 of the yeast Saccharomyces cerevisiae has been cloned and sequenced. The MRP-L27 gene codes for 146 amino acids and is located on chromosome X. The mature YmL27 protein consists of 130 amino acids - after cleaving the putative mitochondrial signal peptide - with a net charge of +17 and a calculated relative molecular mass of 14,798 Da. The YmL27 protein as well as the yeast mitoribosomal protein YmL31, which had been characterized and its gene (MRP-L31) cloned previously, is essential for mitochondrial function as shown by the inability of gene disrupted mutants for the MRP-L27 or MRP-L31 genes to grow on non-fermentable carbon sources.
利用从纯化的线粒体核糖体蛋白（mt r-蛋白）YmL27的N端氨基酸序列推导的合成寡核苷酸，已克隆并测序了酿酒酵母相应的核基因MRP-L27。MRP-L27基因编码146个氨基酸，位于X染色体上。成熟的YmL27蛋白由130个氨基酸组成（在切割假定的线粒体信号肽后），净电荷为+17，计算相对分子质量为14798 Da。YmL27蛋白以及先前已鉴定并克隆其基因（MRP-L31）的酵母线粒体核糖体蛋白YmL31，对于线粒体功能至关重要，这一点通过MRP-L27或MRP-L31基因的基因破坏突变体无法在非发酵碳源上生长得以证明。
