Leith J T, Harrigan P, Michelson S
Department of Radiation Medicine, Radiation Research Laboratories, Providence, RI.
Cell Prolif. 1991 Nov;24(6):587-92. doi: 10.1111/j.1365-2184.1991.tb01187.x.
In this report we have analysed data published in 1989 by Inui et al. (Incidence of precancerous foci of mammary glands and growth rate of transplantable mammary cancers in sialoadenectomized mice. J. Natl Cancer Inst. 81, 1660) involving the effects of perturbation of the epidermal growth factor (EGF) status of mammary tumour-bearing mice on subsequent volumetric responses. Removal of an endogenous EGF stimulus by surgical ablation of the submaxillary glands, the major EGF-producing organ in mice, produced significantly slower growth of rodent mammary neoplasms, decreased success rate of transplantation, and an increase in the latent period before growth occurred. Administration of i.p. EGF (5 micrograms/mouse/day) to sialadectomized tumour-bearing mice would however, increase tumour growth rate. Data were analysed using the Verhulst equation which indicated that the observed effects on tumour volumetrics by either sialoadenectomy or EGF administration could be interpreted as being produced through paracrine pathways. The use of the Verhulstian analysis indicates that it is possible to analyse neoplastic responses and infer whether paracrine or autocrine pathways are involved.
在本报告中，我们分析了犬井等人于1989年发表的数据（涎腺切除小鼠乳腺的癌前病灶发生率及可移植性乳腺癌的生长速率。《国家癌症研究所杂志》81卷，第1660页），这些数据涉及荷乳腺肿瘤小鼠的表皮生长因子（EGF）状态的扰动对后续体积反应的影响。通过手术切除小鼠主要的EGF产生器官——下颌下腺来去除内源性EGF刺激，会使啮齿动物乳腺肿瘤的生长显著减慢，移植成功率降低，且肿瘤生长前的潜伏期延长。然而，给涎腺切除的荷瘤小鼠腹腔注射EGF（5微克/小鼠/天）会提高肿瘤生长速率。使用逻辑斯蒂方程对数据进行分析，结果表明，涎腺切除或EGF给药对肿瘤体积的观察效应可解释为通过旁分泌途径产生。逻辑斯蒂分析的应用表明，有可能分析肿瘤反应并推断是否涉及旁分泌或自分泌途径。
