Liu Jianfang, Sun Jin, Wang Yongjun, Liu Xiaohong, Sun Yinghua, Xu Huan, He Zhonggui
Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
J Chromatogr A. 2007 Sep 14;1164(1-2):129-38. doi: 10.1016/j.chroma.2007.06.066. Epub 2007 Jul 5.
The solvation parameter model has been applied to characterize four microemulsion liquid chromatography (MELC) systems and two micellar liquid chromatography (MLC) systems, and utilized to compare the above systems with other physicochemical and biological processes in this study. The microemulsion mobile phases were composed of sodium dodecyl sulfate (SDS), polyoxyethylene (23) lauryl ether (Brij 35), butanol, heptane and phosphate buffer (pH 7.0) at the designated ratios. The results showed the main difference between the concerned MELC and MLC systems was the decrease of hydrogen-bond basicity of stationary phase with the addition of heptane in microemulsion. Principal component analysis with normalized coefficients can provide consistent results involving the similarities among various systems with that obtained by distance parameter d. Except for some proven similarities of chromatographic systems to octanol-water partition coefficients (logP) and human skin permeation (logK(p)), a microemulsion HPLC system, the mobile phase being 3.3% SDS-6.6% butanol-1.6% heptane-88.5% buffer, was found very similar to drug penetration across blood-brain barrier and its predictive capability for this biological process was originally evaluated in this study.
在本研究中，溶剂化参数模型已被用于表征四个微乳液液相色谱（MELC）系统和两个胶束液相色谱（MLC）系统，并用于将上述系统与其他物理化学和生物过程进行比较。微乳液流动相由十二烷基硫酸钠（SDS）、聚氧乙烯（23）月桂醚（Brij 35）、丁醇、庚烷和指定比例的磷酸盐缓冲液（pH 7.）组成。结果表明，相关的MELC和MLC系统之间的主要差异在于微乳液中加入庚烷后固定相氢键碱度的降低。使用归一化系数的主成分分析可以提供与通过距离参数d获得的结果一致的涉及各种系统之间相似性的结果。除了色谱系统与辛醇 - 水分配系数（logP）和人体皮肤渗透（logK(p)）的一些已证实的相似性外，还发现一种流动相为3.3% SDS – 6.6% 丁醇 – 1.6% 庚烷 – 88.5% 缓冲液的微乳液HPLC系统与药物穿过血脑屏障的过程非常相似，并且在本研究中首次评估了其对该生物过程的预测能力。
