Deane Charlotte M, Dong Mingqiang, Huard Fabien P E, Lance Braddon K, Wood Graham R
Department of Statistics, 1 South Park Road, Oxford University, Oxford OX1 3TG, UK.
Bioinformatics. 2007 Jul 1;23(13):i142-8. doi: 10.1093/bioinformatics/btm175.
Experimentalists have amassed extensive evidence over the past four decades that proteins appear to fold during production by the ribosome. Protein structure prediction methods, however, do not incorporate this property of folding. A thorough study to find the fingerprint of such sequential folding is the first step towards using it in folding algorithms, so assisting structure prediction.
We explore computationally the existence of evidence for cotranslational folding, based on large sets of experimentally determined structures in the PDB. Our perspective is that cotranslational folding is the norm, but that the effect is masked in most classes. We show that it is most evident in alpha/beta proteins, confirming recent findings. We also find mild evidence that older proteins may fold cotranslationally. A tool is provided for determining, within a protein, where cotranslation is most evident.
在过去的四十年里，实验人员积累了大量证据，表明蛋白质似乎在核糖体产生过程中进行折叠。然而，蛋白质结构预测方法并未纳入这种折叠特性。彻底研究以找到这种顺序折叠的指纹，是将其应用于折叠算法从而辅助结构预测的第一步。
我们基于蛋白质数据银行（PDB）中大量实验确定的结构，通过计算探索共翻译折叠证据的存在情况。我们的观点是，共翻译折叠是常态，但在大多数类别中这种效应被掩盖了。我们表明，这种现象在α/β蛋白质中最为明显，这证实了最近的研究发现。我们还发现了一些微弱的证据，表明较古老的蛋白质可能会进行共翻译折叠。我们提供了一种工具，用于确定蛋白质中共翻译最明显的位置。
