Guha A K, Ghose T, Singh M, Aquino J, Blair A H, Luner S J, Mammen M
Department of Pathology, Dalhousie University, Halifax, N.S., Canada.
Cancer Lett. 1991 Dec 9;61(1):35-43. doi: 10.1016/0304-3835(91)90074-r.
We investigated the localization of intravenously injected DAL K45 and DAL K29, two monoclonal antibodies (MABs) against human renal cell carcinoma (RCC), and their F(ab)2 fragments in nude mice bearing intrarenal transplants of the RCC line Caki-1. More of the MABs or their F(ab)2s specifically localized in the tumor than in any normal tissue with the exception of blood. Compared to parent MABs, F(ab)2s were cleared faster from all tissues. In serum, the MABs and F(ab)2s showed a single radioactive peak retaining partial immunoreactivity. DAL K45-F(ab)2 showed the highest tumor:normal tissue localization ratios and the most distinct gamma-camera image at 24 h.
我们研究了静脉注射的两种抗人肾细胞癌（RCC）的单克隆抗体（MABs）DAL K45和DAL K29及其F(ab)2片段在携带RCC细胞系Caki-1肾内移植瘤的裸鼠中的定位情况。除血液外，更多的单克隆抗体或其F(ab)2片段特异性地定位于肿瘤而非任何正常组织。与亲本单克隆抗体相比，F(ab)2片段从所有组织中清除得更快。在血清中，单克隆抗体和F(ab)2片段显示出一个保留部分免疫反应性的单一放射性峰。DAL K45-F(ab)2在24小时时显示出最高的肿瘤与正常组织定位比率以及最清晰的γ相机图像。
