Gershtein Elena S, Scherbakov Alexander M, Shatskaya Valentina A, Kushlinsky Nicolai E, Krasil'nikov Mikhail A
Laboratory of Clinical Biochemistry, Institute of Clinical Oncology, Russian N. N. Blokhin Cancer Research Centre RAMS, 115478 Moscow, Russia.
Anticancer Res. 2007 Jul-Aug;27(4A):1777-82.
The phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway plays a major role in the regulation of breast cancer growth and survival, but the clinical value of its components in human tumours is unclear.
PI3K was analysed using Western blotting with monoclonal antibodies to the p85 subunit in tumour and adjacent mammary gland samples from 33 breast cancer patients. Activated Akt1 (pAkt1) expression was quantified in 46 sample pairs by a direct sandwich ELISA assay.
Tumour PI3K expression was increased in 79% of the investigated sample pairs and was not associated with the main clinico-pathological features. Only 49% of breast cancers had increased pAkt1, but the frequency of its elevation was positively associated with tumour size and histological grade, and controversially related to estrogen and progesterone receptor status.
Increased PI3K but not pAkt1, expression appears to be a widespread feature of human breast cancer indicating the different roles of the two components of one signalling system.
磷脂酰肌醇3激酶（PI3K）/Akt信号通路在乳腺癌的生长和存活调节中起主要作用，但其各组分在人类肿瘤中的临床价值尚不清楚。
使用针对p85亚基的单克隆抗体，通过蛋白质免疫印迹法分析33例乳腺癌患者肿瘤及相邻乳腺组织样本中的PI3K。通过直接夹心酶联免疫吸附测定法对46对样本中的活化Akt1（pAkt1）表达进行定量。
在79%的研究样本对中，肿瘤PI3K表达增加，且与主要临床病理特征无关。仅49%的乳腺癌患者pAkt1表达增加，但其升高频率与肿瘤大小和组织学分级呈正相关，与雌激素和孕激素受体状态的关系存在争议。
PI3K表达增加而非pAkt1表达增加似乎是人类乳腺癌的一个普遍特征，表明同一信号系统的两个组分发挥不同作用。
