Wiest Irmi, Schulze Sandra, Kuhn Christina, Seliger Christian, Hausmann Roland, Betz Peter, Mayr Doris, Friese Klaus, Jeschke Udo
Department of Obstetrics and Gynaecology, LMU Munich, Munich, Germany.
Anticancer Res. 2007 Jul-Aug;27(4A):1981-8.
Sialyl Lewis x (SLeX), sialyl Lewis a (SLeA), Lewis Y (LeY) and the Thomsen-Friedenreich (TF) antigen are carbohydrate motifs that mediate the adhesion between tumour cells and the endothelium. These antigens are usually not expressed in non-malignant tissue. Overexpression of SLeX and SLeA is combined with poor prognosis and malignant relapse. In this study, we analysed the combined expression of SLeX, SLeA, LeY and TF in normal squamous epithelium tissue of the penis shaft, glans and foreskin and in addition of the vagina and vulva.
Paraffin-embedded slides of vaginal tissue (8), vulva tissue (8) and penis shaft (8) and glans tissue (8) were fixed and incubated with monoclonal antibodies against SLeX (IgM), SLeA (IgM), LeY (IgM) and TF (IgM). Staining reaction was performed with ABC reagent. The intensity of immunohistochemical reaction on images of the slides was analyzed using a semiquantitative score.
Strong focal expression of both sialyl Lewis antigens was found in the uretra of the penis shaft and on epithelial tissue of the glans, and permanent moderate expression of SLeX and SLeA in squamous epithelial tissue of the vagina. Moderate expression of TF was observed in male squamous epithelial tissues of the glans and foreskin and faint expression of TF was found in vulval epithelial tissue. Faint expression of Le Y was observed in female vulval epithelial tissue.
Expression of SLeX, SLeA, LeY and especially of the TF antigen in normal non malignant epithelial tissue is surprising and can be explained by the function of this tissue in human reproduction. In addition, moderate TF expression seems to be restricted to epithelial tissue of the penis glans and foreskin.
唾液酸化路易斯x（SLeX）、唾液酸化路易斯a（SLeA）、路易斯Y（LeY）和汤姆森-弗里德赖希（TF）抗原是介导肿瘤细胞与内皮细胞之间黏附的碳水化合物基序。这些抗原通常不在非恶性组织中表达。SLeX和SLeA的过表达与预后不良和恶性复发相关。在本研究中，我们分析了SLeX、SLeA、LeY和TF在阴茎体、龟头和包皮以及阴道和外阴的正常鳞状上皮组织中的联合表达情况。
将阴道组织（8例）、外阴组织（8例）、阴茎体组织（8例）和龟头组织（8例）的石蜡包埋切片固定，并用抗SLeX（IgM）、抗SLeA（IgM）、抗LeY（IgM）和抗TF（IgM）的单克隆抗体进行孵育。用ABC试剂进行染色反应。使用半定量评分分析切片图像上免疫组化反应的强度。
在阴茎体的尿道和龟头的上皮组织中发现两种唾液酸化路易斯抗原均有强局灶性表达，在阴道鳞状上皮组织中SLeX和SLeA呈持续中度表达。在龟头和包皮的男性鳞状上皮组织中观察到TF中度表达，在外阴上皮组织中发现TF微弱表达。在女性外阴上皮组织中观察到LeY微弱表达。
SLeX、SLeA、LeY尤其是TF抗原在正常非恶性上皮组织中的表达令人惊讶，这可以通过该组织在人类生殖中的功能来解释。此外，TF中度表达似乎仅限于阴茎龟头和包皮的上皮组织。
