He Yan, Zhang Lei, Li Ying-jie, Ye Fei, Jiang Yang, Jin Xiao-ming
Department of Pathology, Harbin Medical University, Harbin 150081, China.
Zhonghua Yi Xue Za Zhi. 2007 Apr 3;87(13):909-12.
To study the expression of maspin protein and the correlation between maspin and P53 proteins in gastric carcinoma, so as to provide a new valid index for diagnosis of gastric carcinoma.
Western blotting, immunofluorescence test, and immunohistochemistry were conducted in 32 fresh specimens of gastric carcinoma tissue and adjacent normal tissues obtained during operation performed from January to December 2005 and 232 specimens of gastric tissues obtained during operation or fibergastroscopy, including 172 specimens of gastric carcinoma, conducted 1998 approximately 1999., to determine the protein expression of maspin and P53. The relationship between the protein expression of maspin and that of P53 was analyzed.
The positive rates of maspin protein in the specimens of fresh gastric carcinoma tissues by Western blotting, immunofluorescence test and immunohistochemistry were 56.3%, 50.0%, and 43.8% respectively without significant differences among these 3 groups (all P>0.05). The positive rates of P53 protein in the specimens of the fresh gastric carcinoma tissues by Western blotting, immunofluorescence test and immunohistochemistry were 46.9%, 40.6%, and 37.5% respectively without significant differences among these 3 groups (all P>0.05). The analysis of the 232 specimens of gastric tissues obtained during operation or fibergastroscopy showed that the higher the differentiation of the gastric tissue and the lower the stage of carcinoma the higher the positive rate of maspin protein. The positive rates of maspin protein of the normal gastric mucosa, tissue with atypical hyperplasia, and tissue of gastric carcinoma were 58.3%, 52.8%, and 40.1% respectively with significant differences among these 3 groups (all P<0.05). The positive rate of maspin protein of the tissue with lymph node metastasis was 28.8%, significantly lower than that of the tissues without lymph node metastasis (64.8%, P<0.05). The positive rate of maspin protein of the patients surviving for more than 3 years was 63.5%, significantly higher than that of the patients surviving for 3 years or less (29.6%, P<0.05). Spearman rank correlation test showed a negative correlation between the protein expression of maspin and that of P53 (P<0.01).
The expression of maspin protein is negatively correlates with that of P53 protein. Detection of both maspin and P53 can serve as a prognostic predictor of clinical outcome in the patients with gastric carcinoma.
研究抑癌蛋白maspin在胃癌组织中的表达及其与P53蛋白的相关性，为胃癌的诊断提供新的有效指标。
采用蛋白质印迹法、免疫荧光法及免疫组织化学法，检测2005年1月至12月手术获取的32例新鲜胃癌组织及其癌旁正常组织，以及1998年至1999年手术或纤维胃镜获取的232例胃组织标本（其中胃癌组织172例）中maspin和P53蛋白的表达，并分析maspin与P53蛋白表达的关系。
新鲜胃癌组织标本中，蛋白质印迹法、免疫荧光法及免疫组织化学法检测maspin蛋白的阳性率分别为56.3%、50.0%和43.8%，3组间差异无统计学意义（均P>0.05）；检测P53蛋白的阳性率分别为46.9%、40.6%和37.5%，3组间差异无统计学意义（均P>0.05）。对手术或纤维胃镜获取的232例胃组织标本分析显示，胃组织分化程度越高、癌分期越低，maspin蛋白阳性率越高。正常胃黏膜、不典型增生组织及胃癌组织中maspin蛋白阳性率分别为58.3%、52.8%和40.1%，3组间差异有统计学意义（均P<0.05）。有淋巴结转移组织中maspin蛋白阳性率为28.8%，明显低于无淋巴结转移组织（64.8%，P<0.05）。生存3年以上患者maspin蛋白阳性率为63.5%，明显高于生存3年及以下患者（29.6%，P<0.05）。Spearman等级相关检验显示，maspin与P53蛋白表达呈负相关（P<0.01）。
maspin蛋白表达与P53蛋白表达呈负相关。联合检测maspin和P53可作为胃癌患者临床预后的预测指标。
