Wang Jun, Fan Jun-Ming, Yu Zhen, Wang Yu-Sheng, Zhou Hong-Yan
Henan Academy of Chinese Medicine, Zhengzhou 450004, China.
Zhen Ci Yan Jiu. 2007 Apr;32(2):98-101.
To observe the effect of electroacupuncture (EA) on serum nitric oxide (NO) and plasma endothelin (ET) contents in cerebral ischemia/reperfusion (CI/R) rats.
Fifty-five SD rats were evenly randomized into sham-operation (sham-op), model, EA, acupoint-control (acu-con) and non-acupoint (non-acu)-con groups. CI/R model was produced by occlusion of the basilar artery and bilateral common carotid arteries and reperfusion. EA (10 Hz, 1.5-3 V, duration of 0.6 ms) was applied to left "Jianyu" (LI 15), "Waiguan" (TE 5), etc. for EA group, left "Qinglengyuan" (TE 11), "Lingdao" (HT 4), etc. for acu-con (being not used for CI in clinic) group, and the mid-points between left "Tianquan" (PC 2) and "Quze" (PC 3), PC3 and "Ximen" (PC 4), etc. for non-acu-con group. Carotid venous blood was collected 30 min after CI and after CI/ R respectively for analyzing serum NO content with nitrate reductase (NR) method and plasma ET content with radioimmunoassay, and the brain was taken for detecting its water content and Ca2+ content with flame atomic absorption method.
Compared with sham-op group, serum NO contents of model group 30 min after CI and CI/R, that of EA group 30 min after CI, acu-con group 30 min after CI/R decreased significantly (P < 0.05), and plasma ET content of model group 30 min after CI/R increased considerably (P < 0.05). While compared with model group, serum NO content of EA group 30 min after CI/R increased significantly (P < 0.05) and plasma ET of EA group decreased significantly (P < 0.05). Serum NO level of acu-con group 30 min after CI/R was markedly lower than that of EA group at the same time course. In comparison with sham-op group, Ca2+ contents of model group and acu-con group and water cntent ratio of the brain tissue of model group increased significantly (P < 0.05), while compared with model group, Ca2+ content of EA group decreased remarkably (P < 0.05), and Ca2+ of acu-con group was significantly higher than that of EA group (P < 0.05). No significant differences were found between acu-con and non-acu-con groups in the aforementioned indexes (P > 0.05).
EA of commonly-used acupoints can revise CI/R induced decrease in serum NO level and increase in plasma ET and cerebral Ca2+ significantly at the early period of CI/R, which may contribute to its favorable effects in the treatment of CI.
观察电针（EA）对脑缺血/再灌注（CI/R）大鼠血清一氧化氮（NO）和血浆内皮素（ET）含量的影响。
将55只SD大鼠随机分为假手术（sham-op）组、模型组、电针组、穴位对照（acu-con）组和非穴位（non-acu）对照 组。采用基底动脉和双侧颈总动脉阻断再灌注法制备CI/R模型。电针组将电针（10Hz，1.5 - 3V，脉宽0.6ms）施加于左侧“肩髃”（LI 15）、“外关”（TE 5）等穴位；穴位对照组将电针施加于左侧“清冷渊”（TE 11）、“灵道”（HT 4）等穴位（临床不用于CI治疗）；非穴位对照组将电针施加于左侧“天泉”（PC 2）与“曲泽”（PC 3）、PC 3与 “郄门”（PC 4）等连线中点。分别于CI后30min及CI/R后采集颈静脉血，用硝酸还原酶（NR）法分析血清NO含量，用放射免疫法分析血浆ET含量，并取脑用火焰原子吸收法检测脑组织含水量和Ca2+含量。
与假手术组比较，模型组CI后30min及CI/R后、电针组CI后30min、穴位对照组CI/R后30min血清NO含量显著降低（P < 0.05），模型组CI/R后30min血浆ET含量显著升高（P < 0.05）。与模型组比较，电针组CI/R后30min血清NO含量显著升高（P < 0.05），血浆ET含量显著降低（P < 0.05）。穴位对照组CI/R后30min血清NO水平显著低于电针组同一时间点。与假手术组比较，模型组和穴位对照组Ca2+含量及模型组脑组织含水量比值显著升高（P < 0.05）；与模型组比较，电针组Ca2+含量显著降低（P < 0.05），穴位对照组Ca2+含量显著高于电针组（P < 0.05）。穴位对照组与非穴位对照组上述指标比较差异无统计学意义（P > 0.05）。
常用穴位电针可在CI/R早期明显纠正CI/R所致血清NO水平降低、血浆ET及脑Ca2+升高，这可能是其治疗CI疗效良好的机制之一。
