Wang Shao-Jun, Zhu Bing
Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhen Ci Yan Jiu. 2007 Apr;32(2):119-24.
To investigate the specific acupoint, optimal curative opportunity and the involved pathway of electroacupuncture (EA) for regulating gono-endocrine hormones.
(1) Adult female rats were randomly divided into sham-EA group (n = 32), "Guanyuan" (CV 4) group (n = 36) and "Neiguan" (PC 6) group (n = 36) which were further divided into diestrum, preoestrus (P-EST), estruation and metaoestrus subgroups according to tetra-estrous cycles determined by vaginal smear test. EA (20/60 Hz, 2-3 mA) was applied to CV4 and PC6 separately for 30 min from about 9:30 to 10:00 Am. Blood samples were collected from the common iliac vein before, 30 min and 60 min after EA for detecting serum gonadotropin-releasing hormone (GnRH) and luteotropic hormone (LH) contents with radioimmunoassay. (2) Other female rats were randomly divided into median eminence (ME) lesions group and control group, which were used to observe the influence of hypothalamic ME lesion on the effect of EA of CV4 in regulating GnRH, LH and estradiol (E2) levels.
JD Before the treatment, serum GnRH and LH levels were slightly different in different stages of the estrous cycle, GnRH level was highest in the estruation and LH content highest in the preoestrus. (2) After EA, both serum GnRH and LH levels increased to a certain degree at most stages. Compared with sham-EA group, GnRH levels in the diestrum and metaoestrus and LH in all the 4 stages of the estrous cycle in CV4 group increased significantly (P < 0.05, 0.01). Self-comparison showed that after EA of CV4, serum GnRH level in diestrum and metaoestrum, and LH contents in diestrum and metaoestrus increased markedly (P < 0.05, 0.01); while after EA of PC6, serum GnRH and LH levels in diestrum and metaoestrum increased slightly, suggesting that the effect of EA of CV4 was superior to that of EA of PC6 in promoting secretion of GnRH and LH. (Thirty minutes after EA, self-comparison between pre-and post-EA in each group and comparison among 3 groups showed no significant differences in both serum GnRH and LH levels (P > 0.05). (4) Electrolytic lesion weakened the effects of EA in promoting the secretion of GnRH, LH and E2, though the influence of this lesion on GnRH secretion was milder.
EA can enhance the secretion of GnRH and LH in the diestrum and metaoestrus of the estrous cycle, which is realized by the hypothalamus-pituitary-gonad axis. The effect of EA of CV4 is better than that of PC6.
探讨电针调节性腺内分泌激素的特定穴位、最佳治疗时机及相关途径。
（1）成年雌性大鼠随机分为假电针组（n = 32）、“关元”（CV 4）组（n = 36）和“内关”（PC 6）组（n = 36），根据阴道涂片试验确定的四期发情周期，进一步分为动情间期、动情前期（P - EST）、动情期和动情后期亚组。于上午9:30至10:00分别对CV4和PC6施加电针（20/60 Hz，2 - 3 mA）30分钟。在电针前、电针后30分钟和60分钟从髂总静脉采集血样，采用放射免疫法检测血清促性腺激素释放激素（GnRH）和促黄体生成素（LH）含量。（2）将其他雌性大鼠随机分为正中隆起（ME）损毁组和对照组，观察下丘脑ME损毁对CV4电针调节GnRH、LH和雌二醇（E2）水平作用的影响。
（1）治疗前，发情周期不同阶段血清GnRH和LH水平略有差异，GnRH水平在动情期最高，LH含量在动情前期最高。（2）电针后，多数阶段血清GnRH和LH水平均有一定程度升高。与假电针组相比，CV4组动情间期和动情后期的GnRH水平以及发情周期所有4个阶段的LH水平均显著升高（P < 0.05，0.01）。自身比较显示，CV4电针后，动情间期和动情后期血清GnRH水平以及动情间期和动情后期LH含量显著升高（P < 0.05，0.01）；而PC6电针后，动情间期和动情后期血清GnRH和LH水平略有升高，提示CV4电针在促进GnRH和LH分泌方面效果优于PC6电针。（3）电针后30分钟，各组电针前后自身比较及3组间比较，血清GnRH和LH水平均无显著差异（P > 0.05）。（4）电解损毁减弱了电针促进GnRH、LH和E2分泌的作用，尽管该损毁对GnRH分泌的影响较轻。
电针可增强发情周期动情间期和动情后期GnRH和LH的分泌，这是通过下丘脑 - 垂体 - 性腺轴实现的。CV4电针的效果优于PC6电针。
