Tseng Min, Hoang Kim-Chi, Yang Mei-Kwei, Yang Shu-Feng, Chu Wen Shen
Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331, Hsinchu 300, Taiwan, ROC.
Biodegradation. 2007 Oct;18(5):579-83. doi: 10.1007/s10532-006-9089-z. Epub 2007 Jul 25.
Thermophilic actinomycetes strains were isolated from various environment in Taiwan and screened for degradation of poly(ethylene succinate) (PES), poly(epsilon-caprolactone) (PCL) and/or poly(beta-hydroxybutyrate) (PHB) by the clear-zone method. Out of 341 strains of thermophilic actinomycetes, 105 isolates were PHB-degraders (30.8%), 198 isolates were PCL-decomposers (58.1%), and 99 isolates could degrade PES (29.0%). Furthermore, 77 isolates could degrade both PHB and PCL (22.6%), 35 isolates could degrade both PHB and PES (10.3%), 81 isolates could degrade both PES and PCL (23.8%) and 31 isolates could degrade the three polyesters used in this study (9.1%). Base on the morphological and chemical characteristics, these 31 isolates belonging to Actinomadura (12.9%), Microbispora (25.8%), Streptomyces (48.4%), Thermoactinomyces (9.7%) and Saccharomonospora genus (3.22%).
从台湾的各种环境中分离出嗜热放线菌菌株,并通过透明圈法筛选其对聚琥珀酸乙二酯(PES)、聚己内酯(PCL)和/或聚(β-羟基丁酸酯)(PHB)的降解能力。在341株嗜热放线菌中,105株为PHB降解菌(30.8%),198株为PCL分解菌(58.1%),99株可降解PES(29.0%)。此外,77株可同时降解PHB和PCL(22.6%),35株可同时降解PHB和PES(10.3%),81株可同时降解PES和PCL(23.8%),31株可降解本研究中使用的三种聚酯(9.1%)。根据形态和化学特征,这31株菌株分别属于马杜拉放线菌属(12.9%)、小双孢菌属(25.8%)、链霉菌属(48.4%)、嗜热放线菌属(9.7%)和糖单孢菌属(3.22%)。