Hoang Kim-Chi, Tseng Min, Shu Wei-Jye
Department of Chemical and Material Engineering, Ta-Hwa Institute of Technology, 1 Ta-Hwa Road, Chiung-Lin, Hsinchu 30743, Taiwan, ROC.
Biodegradation. 2007 Jun;18(3):333-42. doi: 10.1007/s10532-006-9067-5. Epub 2006 Nov 16.
Thermophilic actinomycetes were isolated from sediment of the Chingshuei hot spring in north Taiwan, and the strain HS 45-1 was selected from colonies which formed distinct clear zones on agar plate with emulsified polyethylene succinate (PES). The film of PES disappeared within 6 days in liquid cultures at 50 degrees C. The strain HS 45-1 was also able to degrade poly (epsilon-carpolactone) (PCL) and poly (3-hydroxybutyrate) (PHB) films completely within 6 days in liquid cultures. Basing on the results of phynotypic characteristics, phylogenetic studies and DNA-DNA hybridization, strain HS 45-1 should be assigned to Micorbispora rosea subsp. taiwanensis.
从台湾北部清水温泉的沉积物中分离出嗜热放线菌,并从在含有乳化聚琥珀酸乙二酯(PES)的琼脂平板上形成明显透明圈的菌落中挑选出菌株HS 45-1。在50摄氏度的液体培养物中,PES膜在6天内消失。菌株HS 45-1在液体培养物中也能够在6天内完全降解聚(ε-己内酯)(PCL)和聚(3-羟基丁酸酯)(PHB)膜。基于表型特征、系统发育研究和DNA-DNA杂交的结果,菌株HS 45-1应归为玫瑰小双孢菌台湾亚种。