Matsuo Toshihiro, Hiyama Eiso, Sugita Takashi, Shimose Shoji, Kubo Tadahiko, Mochizuki Yu, Adachi Nobuo, Kojima Kensaku, Sharman Patrick, Ochi Mitsuo
Department of Orthopaedic Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Ann Surg Oncol. 2007 Oct;14(10):2896-902. doi: 10.1245/s10434-007-9391-y. Epub 2007 Jul 25.
A giant cell tumor of bone (GCT) is a histologically benign neoplasma that has an unpredictable pattern of biological aggressiveness. In the present study, we investigated whether there was a correlation between telomere length or the levels of telomerase activity and other clinical features of GCTs, for the possible use of these factors as parameters of aggressiveness or prognosis.
In 16 surgically resected GCTs specimens, telomere length was assessed by terminal restriction fragments by Southern blot analysis. Telomerase activity was measured by a semiquantitative polymerase chain reaction-based telomeric repeat amplification protocol assay.
Telomere length reduction was observed in 69% of the GCT samples. The telomere lengths of tumors were significantly shorter than those of normal tissue (P = .008). The mean telomere length of grade 3 tumors was significantly shorter than those of grade 1 and 2 tumors (P = .038). Telomerase activity was detected in 81% of tumor samples. The level of telomerase activity in tumors with local recurrence was significantly higher than in tumors without local recurrence (P = .011).
These results suggest that telomere length correlates with roentgenographic grade as a result of the frequency of cell division, and high telomerase activity indicates the aggressiveness of GCTs.
骨巨细胞瘤（GCT）是一种组织学上为良性的肿瘤，但其生物学侵袭模式难以预测。在本研究中，我们调查了端粒长度或端粒酶活性水平与GCT的其他临床特征之间是否存在相关性，以便将这些因素用作侵袭性或预后的参数。
在16例手术切除的GCT标本中，通过Southern印迹分析的末端限制片段评估端粒长度。通过基于半定量聚合酶链反应的端粒重复序列扩增协议测定法测量端粒酶活性。
69%的GCT样本中观察到端粒长度缩短。肿瘤的端粒长度明显短于正常组织（P = .008）。3级肿瘤的平均端粒长度明显短于1级和2级肿瘤（P = .038）。81%的肿瘤样本中检测到端粒酶活性。局部复发肿瘤的端粒酶活性水平明显高于无局部复发的肿瘤（P = .011）。
这些结果表明，由于细胞分裂频率，端粒长度与X线分级相关，而高端粒酶活性表明GCT具有侵袭性。
